团头鲂SOCS家族基因的分子特征及其对嗜水气单胞菌胁迫的响应

doi:10.13300/j.cnki.hnlkxb.2022.06.026

首页 > 过刊浏览>2022年第41卷第6期 >222-229. DOI:10.13300/j.cnki.hnlkxb.2022.06.026

团头鲂SOCS家族基因的分子特征及其对嗜水气单胞菌胁迫的响应
DOI:
                        10.13300/j.cnki.hnlkxb.2022.06.026
                    
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作者:
                        翟文娅翟文娅
华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/ 长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
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李博李博
华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/ 长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
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王济秀王济秀
华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/ 长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
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王焕岭王焕岭
华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/ 长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
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刘红刘红
华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/ 长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
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作者单位:华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/ 长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
作者简介:翟文娅，E-mail：2021809400@qq.com
通讯作者:刘红，E-mail：liuhong59@mail.hzau.edu.cn
中图分类号:Q959.46+8
基金项目:国家自然科学基金项目（31972781）；现代农业产业技术体系国家大宗淡水鱼产业技术体系建设专项（CARS-45-01）

Molecular characterization of SOCS family genes in Megalobrama amblycephala and their responses to Aeromonas hydrophila infection

Author:

ZHAI Wenya
ZHAI Wenya
College of Fisheries， Huazhong Agricultural University/ Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs/ Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education， Wuhan 430070， China
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LI Bo
LI Bo
College of Fisheries， Huazhong Agricultural University/ Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs/ Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education， Wuhan 430070， China
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WANG Jixiu
WANG Jixiu
College of Fisheries， Huazhong Agricultural University/ Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs/ Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education， Wuhan 430070， China
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WANG Huanling
WANG Huanling
College of Fisheries， Huazhong Agricultural University/ Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs/ Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education， Wuhan 430070， China
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LIU Hong
LIU Hong
College of Fisheries， Huazhong Agricultural University/ Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs/ Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education， Wuhan 430070， China
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Affiliation:

College of Fisheries， Huazhong Agricultural University/ Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs/ Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education， Wuhan 430070， China

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摘要:

为探究团头鲂细胞因子信号转导抑制因子（suppressor of cytokine signaling，SOCS）家族基因的序列特征和功能，从NCBI数据库获取socs1、socs2、socs3a、socs3b、socs4、socs5a、socs5b、socs6、socs7、socs9共10个socs基因的cDNA序列，利用ExPASy、SMART等在线网站对其进行生物信息学分析，预测团头鲂10个SOCS的理论分子质量、理论等电点与结构域等分子特性。系统进化（MEGA 6软件）分析结果显示，10个SOCS可分为2个亚族：Ⅰ型亚族包括SOCS4、SOCS5a、SOCS5b、SOCS6、SOCS7与SOCS9，Ⅱ型亚族包括SOCS1、SOCS2、SOCS3a与SOCS3b。半定量PCR结果显示，健康团头鲂10个socs基因在被检测组织中具有明显的组织特异性，socs1、socs2、socs3在多个组织中表达量较高。在嗜水气单胞菌感染后，采用实时定量PCR检测团头鲂socs基因在脾脏、体肾、头肾中的表达量，其中socs1、socs2、socs3a、socs3b表达量均显著上调。结果表明，团头鲂SOCS家族基因的表达模式各不相同，预示其功能的多样性，其中socs1、socs2、socs3在免疫应答中发挥重要作用。

关键词:团头鲂;嗜水气单胞菌;socs基因;细菌性败血症;抗细菌感染;免疫应答

Abstract:

Suppressor of cytokine signaling （SOCS） plays an important role in inhibiting cytokine receptors in the signaling pathway of JAK/STAT. The cDNA sequences of 10 socs genes including socs1，socs2，socs3a，socs3b，socs4，socs5a，socs5b，socs6，socs7 and socs9 were obtained from the transcriptome of Megalobrama amblycephala to study the sequence characteristics and function of SOCS family genes in M. amblycephala. The theoretical molecular weight， theoretical isoelectric point and functional domain of 10 SOCS in M. amblycephala were predicted with bioinformatics using online websites including ExPASY and SMART. Phylogenetic analysis showed that 10 SOCS were divided into type I subfamily including SOCS4，SOCS5a，SOCS5b，SOCS6，SOCS7 and SOCS9， and type Ⅱ subfamily including SOCS1，SOCS2，SOCS3a and SOCS3b. The results of semi-quantitative RT-PCR showed that the 10 socs genes in healthy M. amblycephala had obvious tissue specificity in the tissues tested. The expression levels of socs1， socs2 and socs3 were high in most tissues tested， followed by socs9， while the expression of other genes was low in all tissues tested. The relative expressions of 10 socs genes in spleen， trunk kidney and head kidney of M. amblycephala were detected after infected with Aeromonas hydrophila. Results showed that the relative expressions of socs1， socs2， socs3a and socs3b were up-regulated significantly at certain time point. It is indicated that the expression patterns of the 10 socs genes in M. amblycephala are different， the functions of the 10 socs genes in M. amblycephala are diverse， and some genes including socs1， socs2， socs3 play an important role genes in immune response. It will lay a foundation for further studying their function.