首页 > 过刊浏览>2022年第41卷第6期 >208-216. DOI:10.13300/j.cnki.hnlkxb.2022.06.024
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黄鳝Ⅰ型α螺旋细胞因子中6个白介素基因的序列特征及表达分析
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作者:
作者单位:

1.长江大学教育部湿地生态与农业利用工程研究中心,荆州 434024;2.内江师范学院生命科学学院/长江上游鱼类资源保护与利用四川省重点实验室,内江 641100

作者简介:

谢梦， E-mail：287896962@qq.com

通讯作者:

许巧情， E-mail：xuqiaoqing@163.com

中图分类号:

Q786

基金项目:

长江大学教育部湿地生态与农业利用工程研究中心开放课题（KF202114）；农业农村部长江中上游渔业资源与环境科学开放项目（FRESES201901）；农业农村部长江渔业资源与环境调查项目（CJDC-2017-09）


Sequence characterization and expression analysis of six interleukin genes of type I α-helix cytokines in Monopterus albus
Author:
Affiliation:

1.Engineering Research Center of Ecology and Agricultural Use of Wetland， Yangtze University，Jingzhou 434024，China;2.Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province/College of Life Sciences， Neijiang Normal University，Neijiang 641100，China

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    摘要:

    为了解白介素在黄鳝抗维氏气单胞菌（Aeromonas veronii）感染过程中的分子机制，利用生物信息学方法分析了黄鳝il-6、il-12p35、il-12p40、il-15、ebi3和il-34基因及其编码蛋白的序列特征，并采用实时荧光定量PCR（qRT-PCR）方法分析了这些基因在健康和维氏气单胞菌感染后黄鳝中的表达模式。结果显示，黄鳝6个白介素蛋白氨基酸序列与尼罗罗非鱼相似性最高且进化关系最近；6个白介素基因与其他鱼类的基因结构基本相同；共线性分析显示il-15在进化上相对保守；白介素基因在健康黄鳝不同组织中均有表达；与对照组相比，白介素基因的表达在维氏气单胞菌感染后均发生了显著变化，其中，il-6、il-12p35和ebi3参与早期阶段的黄鳝抗菌免疫反应。研究结果表明，il-6、il-12p35、il-12p40、il-15、ebi3和il-34基因在黄鳝抵抗病原入侵的免疫防御活动中均有调控作用。

    Abstract:

    To understand the molecular mechanism of interleukin in Monopterus albus against Aeromonas veronii infection，the sequence characteristics of six interleukin genes including il-6，il-12p35，il-12p40，il-15，ebi3 and il-34 and their coded proteins in M. albus were analyzed by bioinformatics method，and the expression patterns of these genes in healthy and A. veronii infected M. albus were analyzed by real-time quantitative PCR （qRT-PCR）. The results showed that the amino acids sequences of six interleukin proteins had the highest similarity and the closest evolutionary relationship with those of tilapia. The gene structure of six interleukin genes was basically the same as that of other fish. Synteny analysis showed that il-15 was relatively conserved in evolution. Interleukin genes were expressed in different tissues of healthy M. albus. Compared with the control group，the expression of interleukin genes changed significantly after A. veronii infection. Among them，il-6，il-12p35 and ebi3 were involved in the early stage of antibacterial immune response of M. albus. The results showed that il-6，il-12p35，il-12p40，il-15，ebi3 and il-34 genes all played a regulatory role in the immune defense of M. albus against pathogen invasion.

    表 3 脊椎动物与黄鳝6个白介素蛋白的相似性比较Table 3 Similarity comparison between vertebrate six interleukin proteins
    表 2 黄鳝6个白介素基因序列特征Table 2 Sequence characterization of six interleukins in swamp eel
    图1 黄鳝和其他物种中6个白介素蛋白的系统进化树Fig.1 Phylogenetic trees of six interleukin proteins in swamp eel and other species
    图2 黄鳝与其他动物6个白介素基因的结构示意图Fig.2 Genomic gene organization of six interleukin genes in swamp eel and other animals
    图3 基因共线性示意图Fig.3 Schematic representation of gene synteny
    图4 健康黄鳝不同组织中6个白介素基因的表达模式Fig.4 Expression pattern of six interleukin genes in various tissue in healthy swamp eel
    图5 6个白介素基因在维氏气单胞菌感染后的表达变化Fig.5 Gene expression of six interleukin genes in response to A. veronii challenges
    表 1 本研究所用的主要引物Table 1 Primers used in the study
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谢梦,张哲华,周江,许巧情,邹远超.黄鳝Ⅰ型α螺旋细胞因子中6个白介素基因的序列特征及表达分析[J].华中农业大学学报,2022,41(6):208-216

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  • 收稿日期:2022-06-19
  • 在线发布日期: 2022-12-09
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