首页 > 过刊浏览>2022年第41卷第6期 >192-200. DOI:10.13300/j.cnki.hnlkxb.2022.06.022
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团头鲂咽齿发育相关基因系统进化及其表达分析
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作者:
  • 朱德杰 1

    朱德杰

    华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/农业动物遗传育种与繁殖教育部重点实验室/湖北省名优鱼育种与健康养殖工程技术研究中心/长江经济带大宗水生生物产业绿色发展 教育部工程研究中心，武汉 430070
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  • 吴亚明 1

    吴亚明

    华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/农业动物遗传育种与繁殖教育部重点实验室/湖北省名优鱼育种与健康养殖工程技术研究中心/长江经济带大宗水生生物产业绿色发展 教育部工程研究中心，武汉 430070
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  • 陈宇龙 1

    陈宇龙

    华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/农业动物遗传育种与繁殖教育部重点实验室/湖北省名优鱼育种与健康养殖工程技术研究中心/长江经济带大宗水生生物产业绿色发展 教育部工程研究中心，武汉 430070
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  • 高泽霞 1,2

    高泽霞

    华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/农业动物遗传育种与繁殖教育部重点实验室/湖北省名优鱼育种与健康养殖工程技术研究中心/长江经济带大宗水生生物产业绿色发展 教育部工程研究中心，武汉 430070;湖北洪山实验室，武汉430070
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作者单位:

1.华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/农业动物遗传育种与繁殖教育部重点实验室/湖北省名优鱼育种与健康养殖工程技术研究中心/长江经济带大宗水生生物产业绿色发展 教育部工程研究中心，武汉 430070;2.湖北洪山实验室，武汉430070

作者简介:

朱德杰，E-mail：515948889@qq.com

通讯作者:

高泽霞，E-mail： gaozx@mail.hzau.edu.cn

中图分类号:

Q786;Q959.46+8

基金项目:

国家大宗淡水鱼类产业技术体系建设项目（CARS-45-01）；国家自然科学基金项目（31872559）；武汉市科技计划项目（2020020601012253）


Phylogeny and expression analysis of genes related to tooth development in Megalobrama amblycephala
Author:
  • ZHU Dejie 1

    ZHU Dejie

    College of Fisheries，Huazhong Agricultural University/ Key Laboratory of Freshwater Animal Breeding，Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics，Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education，Wuhan 430070，China
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  • WU Yaming 1

    WU Yaming

    College of Fisheries，Huazhong Agricultural University/ Key Laboratory of Freshwater Animal Breeding，Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics，Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education，Wuhan 430070，China
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  • CHEN Yulong 1

    CHEN Yulong

    College of Fisheries，Huazhong Agricultural University/ Key Laboratory of Freshwater Animal Breeding，Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics，Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education，Wuhan 430070，China
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  • GAO Zexia 1,2

    GAO Zexia

    College of Fisheries，Huazhong Agricultural University/ Key Laboratory of Freshwater Animal Breeding，Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics，Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education，Wuhan 430070，China;Hubei Hongshan Lab，Wuhan 430070，China
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Affiliation:

1.College of Fisheries，Huazhong Agricultural University/ Key Laboratory of Freshwater Animal Breeding，Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics，Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt， Ministry of Education，Wuhan 430070，China;2.Hubei Hongshan Lab，Wuhan 430070，China

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    摘要:

    为探明鱼类咽齿发生发育的分子机制，以我国特有的经济鱼类团头鲂（Megalobrama amblycephala）为研究对象，通过团头鲂全基因组序列Blast获得团头鲂分泌型钙结合磷蛋白（secretory calciumbinding phosphoprotein，SCPP）家族基因的cDNA序列，采用实时荧光定量的方法比较分析SCPP家族基因在团头鲂咽齿发生发育关键时期第四、第五鳃弓中的表达情况，以及在1龄团头鲂不同组织中的表达情况，筛选调控咽齿发育的关键调控基因。研究结果显示，团头鲂enam、scpp1、ambn、scpp9、odam、spp1与斑马鱼具有较高同源性，进化树中距离较近；通过比对不同脊椎动物enam与odam这2个基因编码的氨基酸序列，发现在鱼类中均存在2段氨基酸序列的缺失。1龄团头鲂不同组织的SCPP家族基因定量表达结果显示，enam、scpp1、ambn、scpp9和spp1在第五鳃弓（有咽齿）和肋骨里面有很高的表达量，与其他组织中的表达水平大多存在显著性差异（P<0.05），且在第四鳃弓（无咽齿）中的表达量最低。团头鲂第四、第五鳃弓早期（9~54 d）咽齿发育阶段定量结果显示enam、ambn、scpp9、odam、spp1在第五鳃弓表达量显著高于第四鳃弓表达量，其中scpp9在第五鳃弓中的表达量极高。以上研究结果表明enam、scpp1、ambn、scpp9、odam、spp1均参与团头鲂咽齿发育的调控，其中scpp9在团头鲂咽齿发育过程中调控作用较为明显，推测其与咽齿发育关系更为密切。

    Abstract:

    In order to clarify the molecular mechanism of fish pharyngeal tooth development，taking Megalobrama amblycephala，a unique economic fish in China，as the research object，the cDNA sequence of secretory calciumbinding phosphoprotein （SCPP） family genes was obtained through whole genome sequence Blast. The expression of SCPP family genes in the fourth and fifth branchial arches during the critical stages of pharyngeal tooth development and in different tissues of one-year-old M. amblycephala was analyzed by qRT-PCR method to screen the key genes regulating the development of pharyngeal teeth. Multiple sequence alignment of amino acids showed that enam，scpp1，ambn，scpp9，odam，and spp1 of M. amblycephala and zebrafish share high identity，which were close to each other in the phylogenetic tree. By comparing the amino acid sequences encoded by enam and odam in different vertebrates，it was found that there were two amino acid sequence deletions in fish. The expression levels of enam，scpp1，ambn，scpp9 and spp1 in the fifth branchial arch （with pharyngeal teeth） and ribs were significantly higher than those in other tissues （P<0.05），and the expression levels of them were lowest in the fourth branchial arch （without pharyngeal teeth）. The expression levels of enam，ambn，scpp9，odam，and spp1 in the fifth branchial arch were significantly higher than those in the fourth branchial arch，and the expression level of scpp9 in the fifth branchial arch was extremely high. The results above showed that enam，scpp1，ambn，scpp9，odam，and spp1 were all involved in the regulation of pharyngeal tooth development，among which，scpp9 played a more important role in the regulation of pharyngeal tooth development，suggesting that it was more closely related to the development of pharyngeal teeth.

    图1 团头鲂SCPP家族基因与其他脊椎动物基因的同源性比较Fig.1 Comparison of homology between SCPP family genes of M. amblycephala and genes of other vertebrates
    图2 团头鲂SCPP家族基因与其他脊椎动物基因的系统进化树Fig.2 Phylogenetic tree of SCPP family genes between M. amblycephala and other vertebrates
    图3 不同脊椎动物enam和odam部分编码序列比对分析Fig.3 Partial alignment sequences of enam and odam in the different vertebrates
    图4 团头鲂SCPP家族基因在不同组织的相对表达量Fig.4 Relative expression of SCPP family genes in the different tissues of M. amblycephala
    图5 团头鲂SCPP家族基因在早期咽齿发育阶段的相对表达量Fig.5 Relative expression of SCPP family genes in the early pharyngeal tooth developmental stages in M. amblycephala
    表 1 基因荧光定量PCR引物信息Table 1 Primer information for real time PCR of genes
    表 2 团头鲂SCPP家族基因的理化性质Table 2 Physical and chemical properties of SCPP family genes in M. amblycephala
    参考文献
    [1] KAWASAKI K，WEISS K M.SCPP gene evolution and the dental mineralization continuum[J].Journal of dental research，2008，87（6）：520-531.
    [2] KAZUHIKO K，AMEMIYA C T.SCPP genes in the coelacanth：tissue mineralization genes shared by sarcopterygians[J].Journal of experimental zoology part B：molecular and developmental evolution，2014，322（6）：390-402.
    [3] SIRE J Y，DAVIT-BEAL T，DELGADO S，et al.First-generation teeth in nonmammalian lineages：evidence for a conserved ancestral character[J].Microscopy research and technique，2002，59（5）：408-434.
    [4] SIRE J Y，HUYSSEUNE A.Formation of dermal skeletal and dental tissues in fish：a comparative and evolutionary approach[J].Biological reviews of the Cambridge Philosophical Society，2003，78（2）：219-249.
    [5] STOCK D W.The genetic basis of modularity in the development and evolution of the vertebrate dentition[J].Philosophical transactions of the Royal Society of London.Series B，biological sciences，2001，356（1414）：1633-1653.
    [6] KAWASAKI K， SUZUKI T， WEISS K M. Phenogenetic drift in evolution： the changing genetic basis of vertebrate teeth[J]. PNAS， 2005， 102：18063-18068.
    [7] CHAI Y，JIANG X，ITO Y，et al.Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis[J].Development （Cambridge，England），2000，127（8）：1671-1679.
    [8] VENKATESH B， LEE A P， RAVI V， et al. Elephant shark genome provides unique insights into gnathostome evolution[J]. Nature， 2014， 505（7482）： 174-179.
    [9] LAUE K，J?NICKE M，PLASTER N，et al.Restriction of retinoic acid activity by Cyp26b1 is required for proper timing and patterning of osteogenesis during zebrafish development[J].Development （Cambridge，England），2008，135（22）：3775-3787.
    [10] LIN Q，FAN S H，ZHANG Y H，et al.The seahorse genome and the evolution of its specialized morphology[J].Nature，2016，540（7633）：395-399.
    [11] 王旭东，唐雨甜，熊雪梅，等.团头鲂scxa基因SNP位点与肌间骨数目的关联性分析[J].华中农业大学学报，2021，40（4）：149-156.WANG X D，TANG Y T，XIONG X M，et al.Expression of scxa and correlation analysis between SNP and number of intermuscular bones in Megalobrama amblycephala[J].Journal of Huazhong Agricultural University，2021，40（4）：149-156（in Chinese with English abstract）.
    [12] 高泽霞，王卫民，蒋恩明，等. 团头鲂种质资源及遗传改良研究进展[J]. 华中农业大学学报，2014，33（3）： 138-144. GAO Z X，WANG W M，JIANG E M，et al. Research advances on germplasm resources and genetic improvement of blunt snout bream （Megalobrama amblycephala）[J]. Journal of Huazhong Agricultural University，2014，33（3）：138-144（in Chinese with English abstract）.
    [13] LIU H，CHEN C H，Lü M L，et al.A chromosome-level assembly of blunt snout bream （Megalobrama amblycephala） genome reveals an expansion of olfactory receptor genes in freshwater fish[J].Molecular biology and evolution，2021，38（10）：4238-4251.
    [14] GANSNER J M，MENDELSOHN B A，HULTMAN K A，et al. Essential role of lysyl oxidases in notochord development[J]. Developmental biology，2007，307（2）：202-213.
    [15] MEREDITH R W，ZHANG G J，GILBERT M T P，et al. Evidence for a single loss of mineralized teeth in the common avian ancestor[J/OL]. Science，2014，346（6215）：1254390[2022-04-25].https：//doi.org/ 10.1126/science.1254390.
    [16] BRAASCH I，GEHRKE A R，SMITH J J，et al.The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons[J].Nature genetics，2016，48（4）：427-437.
    [17] KAWASAKI K. The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues[J]. Development genes and evolution，2009，219（3）：147-157.
    [18] LIU Z J，LIU S K，YAO J，et al.The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts[J/OL].Nature communications，2016，7：11757[2022-04-25]. https：//doi.org/10.1038/ncomms11757.
    [19] LV Y Y，KAWASAKI K，LI J，et al.A genomic survey of SCPP family genes in fishes provides novel insights into the evolution of fish scales[J/OL].International journal of molecular sciences，2017，18（11）：2432[2022-04-25]. https：//doi.org/ 10.3390/ijms18112432.
    [20] KARAGIC N，SCHNEIDER R F，MEYER A，et al.A genomic cluster containing novel and conserved genes is associated with cichlid fish dental developmental convergence[J].Molecular biology and evolution，2020，37（11）：3165-3174.
    [21] KAGUE E，WITTEN P E，SOENENS M，et al.Zebrafish sp7 mutants show tooth cycling independent of attachment，eruption and poor differentiation of teeth[J].Developmental biology，2018，435（2）：176-184.
    [22] ABDUWELI D，BABA O，TABATA M J，et al.Tooth replacement and putative odontogenic stem cell niches in pharyngeal dentition of medaka （Oryzias latipes）[J].Microscopy，2014，63（2）：141-153.
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朱德杰,吴亚明,陈宇龙,高泽霞.团头鲂咽齿发育相关基因系统进化及其表达分析[J].华中农业大学学报,2022,41(6):192-200

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  • 收稿日期:2022-04-25
  • 在线发布日期: 2022-12-09
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