1.华中农业大学水产学院/农业动物遗传育种与繁殖教育部重点实验室/ 农业农村部淡水生物繁育重点实验室，武汉 430070;2.长江经济带大宗水生生物产业绿色发展教育部工程研究中心/ 湖北省名优鱼育种与健康养殖工程技术研究中心，武汉 430070
桂聪，E-mail：1141652948@qq.com
袁勇超，E-mail：yyc@mail.hzau.edu.cn
湖北省重点研发计划项目（2020BBA056）
- GUI Cong 1
- DENG Qiqi 1
- YANG Huijun 1
- MO Aijie 1
- SHEN Zhigang 1
- YUAN Yongchao 1,2
YUAN Yongchao
College of Fisheries，Huazhong Agricultural University/Key Lab of Agricultural Animal Genetics， Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs，Wuhan 430070，China;Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt，Ministry of Education/Hubei Province Aquatic Animal Disease Control Engineering Technology Research Center/Wuhan 430070，China
在期刊界中查找
在百度中查找
在本站中查找
1.College of Fisheries，Huazhong Agricultural University/Key Lab of Agricultural Animal Genetics， Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding， Ministry of Agriculture and Rural Affairs，Wuhan 430070，China;2.Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt，Ministry of Education/Hubei Province Aquatic Animal Disease Control Engineering Technology Research Center/Wuhan 430070，China
- 摘要 |
- 图/表 |
- 访问统计 |
- 参考文献 |
- 相似文献 |
- 引证文献 | |
- 文章评论
为研发优质的低蛋白饲料，以大口黑鲈（Micropterus salmoides）幼鱼为研究对象，设置46%（对照）、41%（LP1）和36%（LP2）3个饲料蛋白质梯度，在41%和36%蛋白饲料的基础上补充相应的晶体赖氨酸与蛋氨酸，使赖氨酸与蛋氨酸含量与对照组保持一致，分别记作LP1+AA组和LP2+AA组。进行周期为8周的饲养试验，试验结束后对大口黑鲈的生长性能和抗氧化能力进行测定。结果显示：LP1+AA组大口黑鲈的增重率、特定生长率、饲料系数和蛋白质保留率与对照组相比无显著性差异，其他各组与对照组相比发生显著性变化。随着蛋白水平降低，肌肉和全鱼中粗蛋白含量均显著降低，但LP1+AA组与对照组之间无显著性差异；粗脂肪和粗灰分含量均无显著性变化。低蛋白日粮组试验鱼的肝体比显著下降，但LP1+AA组与对照组无显著性差异。相比于对照组，LP2组和LP2+AA组试验鱼的肝脏活性氧水平和总抗氧化能力均发生显著性变化；LP1组试验鱼肝脏的总抗氧化能力显著性降低，但补充赖氨酸和蛋氨酸有明显的提高。降低大口黑鲈饲料中的蛋白水平（5%或10%）对肝脏中转氨酶的活性未产生显著影响；LP2组试验鱼血清中谷丙转氨酶活性发生显著变化，但在LP2试验组的基础上添加赖氨酸和蛋氨酸能起到改善作用，并且与对照组相比无显著性差异。结果表明，饲料粗蛋白含量从46%降低至41%并补充适量赖氨酸、蛋氨酸，对大口黑鲈幼鱼生长性能没有显著影响；但饲料粗蛋白含量降低至36%并补充赖氨酸和蛋氨酸，不能有效缓解饲料粗蛋白水平过低对生长性能造成的负面影响。
To investigate the effects of methionine and lysine supplementation on growth performance，transaminase，body composition and antioxidant performance of juvenile Micropterus Salmoides，three dietary protein gradients （46% （control），41% （LP1） and 36% （LP2）） were set.On the basis of 41% and 36% protein diets，crystal lysine and methionine were supplemented to keep the contents of lysine and methionine consistent with the control group，which were recorded as LP1+AA group and LP2+AA group，respectively.Each experimental group contained 3 biological replicates and the feeding period was 8 weeks.The results showed as follows：compared with the control group，there were no significant differences in weight gain rate （WGR），specific growth rate （SGR），feed conversion ratio （FCR） and protein retention （PR） in LP1+AA group，but significant changes were observed in other groups.With the decrease of protein level，crude protein content in muscle and whole body was significantly decreased，but not in LP1+AA group compared with the control group.There was no significant change in crude fat and ash content.The hepatosomatic ratio （HSI） in low protein diet group was significantly decreased，but there was no significant decrease in LP1+AA group compared with the control group.Compared with the control group，the levels of liver reactive oxygen species （ROS） and total antioxidant capacity （T-AOC） of fish in LP2 and LP2+AA groups were significantly changed.The T-AOC of liver in LP1 group was significantly decreased，but it was significantly increased in lysine and methionine supplementation groups.Reducing dietary protein level （5% or 10%） had no significant effect on liver transaminase activity.Although serum glutamic-pyruvic transaminase （GPT） activity changed significantly in LP2 group，there was no significant difference when lysine and methionine were added compared with the control group.The results showed that when dietary protein content was reduced from 46% to 41% and supplemented with essential amino acids，the growth performance was not significantly affected，but when the dietary protein content was reduced to 36%，lysine and methionine supplementation could not effectively alleviate the negative effects of low dietary protein on growth performance.
引用本文
桂聪,邓琦琦,杨慧君,莫爱杰,沈志刚,袁勇超.低蛋白饲料添加蛋氨酸和赖氨酸对大口黑鲈生长性能和抗氧化能力的影响[J].华中农业大学学报,2022,41(6):184-191
复制
文章指标
- 点击次数:
- 下载次数:
- HTML阅读次数:
历史
- 收稿日期:2022-03-12
- 在线发布日期: 2022-12-09
文章二维码
