不同耕作模式对稻田土壤真菌丰度及群落结构的影响
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作者:
作者单位:

1.江西农业大学生态科学研究中心, 南昌 330045;2.中国科学院亚热带农业生态研究所桃源农业生态试验站/亚热带农业生态过程重点实验室,长沙 410125

作者简介:

徐慧芳,E-mail:xhuifang@yeah.net

通讯作者:

黄国勤,E-mail:hgqjxes@sina.com

中图分类号:

S344.1;S511

基金项目:

国家重点研发计划项目(2016YFD0300208); 国家自然科学基金项目(32160310);江西省教育厅科技计划项目(GJJ200444)


Effects of farming patterns on abundance and community structure of fungi in paddy soils
Author:
Affiliation:

1.Center for Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, China;2.Taoyuan Station of Agro-Ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences/Key Laboratory of Agro-Ecological Processes in Subtropical Regions, Changsha 410125, China

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    摘要:

    为分析我国典型的稻田耕作模式下土壤真菌丰度及群落结构差异,以长期(10 a)4种典型稻田耕作模式为研究对象,设置以下处理:双季稻(DR)、早稻-晚稻(MR)、中稻-油菜(MROR)及中稻-小白菜-油菜(MRPOR),利用实时荧光定量PCR及高通量测序手段测定土壤真菌的丰度及群落组成。结果显示,双季稻转变为单季稻及水旱轮作模式后显著改变了真菌的丰度,其中双季稻转变为中稻-小白菜-油菜后显著增加了真菌的丰度,相关性分析发现,硝态氮与真菌的丰度呈显著的正相关关系;双季稻转为单季稻及水旱轮作模式后显著改变了真菌的群落结构,冗余分析发现水分含量是影响真菌群落结构最主要的环境因子。综上研究表明,不同轮作模式下稻田土壤真菌群落丰度、组成及多样性存在差异。不同处理下真菌丰度受土壤硝态氮影响较大,而真菌群落结构主要受土壤含水量的影响。

    Abstract:

    Four farming patterns including double rice (DR),middle rice (MR),middle rice-rapeseed rotation (MROR), and middle rice-pakchoi-rapeseed rotation (MRPOR) in a long-term (10 years) practices were selected to analyze the abundance and differences in community structure of fungi under different farming systems in China. The abundance and community composition of fungi in paddy soils were determined by real-time fluorescence quantitative PCR and high-throughput sequencing to analyze the differences in the abundance and community structure of fungi in soil under different farming patterns and their influencing factors. Results showed that MR, MROR and MRPOR had a significant influence on the abundance of fungi. MRPOR increased the abundance of fungi. The result of correlation analyses showed that nitrate nitrogen was significantly and positively correlated with the abundance of fungi. The crop rotation had a significant influence on the gene community structure of fungi in soil. The result of redundancy analyses showed that the content of soil water was the most significant factor affecting the community structure of fungi. In conclusion, the abundance, composition and diversity of communities of fungi in soil were different under different farming patterns of rice. The abundance of fungi was greatly affected by the content of nitrate nitrogen in soil under different farming patterns, and the community structure of fungi was mainly affected by the content of water in soil.

    图1 不同耕作模式土壤真菌丰度的变化Fig.1 The abundance of soil fungi under different farming patterns
    图2 不同耕作模式土壤真菌的主成分分析PCAFig.2 PCA analysis for soil fungi under different farming patterns
    图3 不同耕作模式土壤真菌群落Venn图Fig.3 Venn of soil fungi under different farming patterns
    图4 不同耕作模式土壤真菌门水平群落组成Fig.4 Composition of soil fungi phylum under different farming patterns
    图5 不同耕作模式土壤真菌群落结构的db-RDA图Fig.5 db-RDA of soil fungi under different farming patterns
    表 2 不同耕作模式下土壤真菌群落的Alpha多样性指数Table 2 The fungi Alpha diversities of different genes among four farming patterns
    表 1 不同耕作模式下稻田施肥状况Table 1 Amount of fertilizer applied of four farming patterns
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徐慧芳,万子维,盛荣,魏文学,张文钊,黄国勤.不同耕作模式对稻田土壤真菌丰度及群落结构的影响[J].华中农业大学学报,2022,41(6):35-41

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  • 收稿日期:2022-03-24
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