The effects of three commonly used herbicides including glyphosate isopropylamine，glufosinate，and quizalofop-p-ethyl on the physicochemical properties of soil，rhizosphere microorganisms in soil and fruit quality in citrus orchards were investigated.The 20-year-old satsuma mandarin fruit trees were used to detect the changes of physicochemical properties of soil，the diversity of rhizosphere microorganisms in soil and fruit quality.The results showed that the spraying of glyphosate isopropylamine led to soil acidification，while the spraying of glufosinate or quizalofop-p-ethyl had no significant effect on soil pH.The spraying of the three herbicides increased the bulk weight of soil and somehow activated acid phosphatase，alkaline phosphatase，urease and sucrase in soil.None of the three herbicides had effect on the nutrient content of organic matter，soil alkaline nitrogen，available potassium and available phosphorus in soil.Spraying the three herbicides led to significant changes of microbial community composition in soil and the decrease in diversity of microbial rhizosphere，resulting in a decrease in the relative abundance of microorganisms including Acidobacteria and Actinobacteria，and an increase in the relative abundance of microorganisms such as Cyanobacteria phylum.The spraying of three herbicides resulted in a decrease in the weight of single fruit and the content of soluble solids，vitamin C，and an increase in the content of titrable acid，and the delayed change of peel color.It is indicated that the spraying of herbicides had significant adverse effects on the ecology of soil and the fruit quality in orange orchards.It will provide a theoretical basis for understanding the function of herbicides objectively and advocating less spraying of herbicides in citrus orchards.