1.华中农业大学园艺植物生物学教育部重点实验室，武汉 430070;2.湖北省当阳市特产技术推广中心，当阳 444100;3.华中农业大学工学院，武汉 430070;4.湖南省邵阳市农业科学院，邵阳 422002
石莹，E-mail：2269878819@qq.com
刘继红，E-mail：liujihong@mail.hzau.edu.cn
S666.105+.1
国家重点研发计划项目（2019YFD1001402）；湖北省重点研发计划项目（2020BBA036；2022BBA0073）；湖北省农业创新行动计划项目
- SHI Ying 1,4
- ZENG Yike 1
- CHEN Siyi 2
- LI Guojing 2
- HUANG Xianbiao 2
- LI Shanjun 3
- LI Chunlong 1
- XIE Zongzhou 1
- LIU Jihong 1
1.Key Laboratory of Horticultural Plant Biology，Ministry of Education，Huazhong Agricultural University，Wuhan 430070，China;2.Dangyang Extension Centre of Technology for Specialty Products，Hubei Province， Dangyang 444100，China;3.College of Engineering，Huazhong Agricultural University，Wuhan 430070，China;4.Shaoyang Academy of Agricultural Sciences，Hunan Province，Shaoyang 422002，China
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为提升椪柑果实品质，以‘鄂柑1号’椪柑为材料，探究机械修剪疏果对果实品质的影响并基于糖酸代谢相关基因表达解析其作用机制。结果显示：机械修剪疏果显著加快了疏果进程，极大地节约了疏果用工；机械修剪疏果显著增大了果实横径、纵径、单果质量和果皮硬度，显著提高成熟期大果比例至57%；机械修剪疏果还加快了果实转色，显著提高果实可溶性固形物含量至11.57%，降低了可滴定酸含量；通过气相色谱法发现机械修剪疏果显著提高果实的蔗糖含量至52.10 mg/g；实时荧光定量PCR分析发现机械修剪疏果的果实中蔗糖合成相关基因和柠檬酸降解相关基因表达量显著上调。研究结果表明，机械修剪可以作为一种省力化的疏果方式，通过促进蔗糖合成基因和柠檬酸降解基因表达来提升椪柑果实的品质。
‘Egan No.1’ Ponkan was used to study the effects of mechanical pruning-mediated fruit thinning on improving fruit quality to find a labor-saving way for thinning fruit in a more efficient manner.At the same time，the expression patterns of genes associated with sucrose and citric acid metabolism were analyzed.The results showed that the fruit thinning speed and efficiency were substantially accelerated by mechanical pruning，leading to significant labor-saving.In addition，mechanical pruning-mediated fruit thinning significantly increased the transverse diameters，longitudinal diameters，single fruit weight and fruit firmness of the Ponkan fruit.Meanwhile，the proportion of large-sized fruit at the stage of ripening was significantly increased to 57%.Mechanical pruning-mediated fruit thinning accelerated the color change of fruit peel，significantly increased total soluble solids to 11.57% and reduced the content of titratable acids.The content of sucrose was significantly increased to 52.10 mg/g.The result of quantitative real-time PCR showed that genes involved in the synthesis of sucrose and the degradation of citric acid were significantly up-regulated in the fruits collected from the thinned trees in comparison with the unthinned trees.Mechanical pruning-mediated fruit thinning can be used as a labor-saving fruit thinning method to improve the quality of Ponkan fruit by up-regulating genes associated with the synthesis of sucrose and the degradation of citric acid.
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石莹,曾译可,陈思怡,李国敬,黄先彪,李善军,李春龙,谢宗周,刘继红.机械修剪疏果提升椪柑果实品质的作用及机制[J].华中农业大学学报,2022,41(5):68-76
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- 收稿日期:2022-02-22
- 在线发布日期: 2022-10-11
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