利用重组自交系群体定位水稻茎粗QTL
作者:
作者单位:

1.华中农业大学生命科学技术学院/作物遗传改良全国重点实验室,武汉430070;2.农业农村部西南水稻生物学与遗传育种重点实验室,德阳618000

作者简介:

朱小平,E-mail: zhuxiaoping@webmail.hzau.edu.cn

通讯作者:

林拥军,E-mail: yongjunlin@mail.hzau.edu.cn

中图分类号:

S511.2+1

基金项目:

国家转基因重大专项(2016ZX08001-001)


Mapping QTLs for internode diameter using a population of recombinant inbred lines
Author:
Affiliation:

1.National Key Laboratory of Crop Genetic Improvement/ College of Biology Science and Technology,Huazhong Agricultural University, Wuhan 430070,China;2.Ministry of Agriculture and Rural Affairs Key Laboratory of Southwest Rice Life and Genetic Breeding,Deyang 618000,China

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    摘要:

    为开展水稻茎粗相关基因的精细定位,利用岗46B/A232的高世代重组自交系群体和基于重测序构建的高密度遗传连锁图谱,采用复合区间作图法(CIM),利用R/qtl软件包对水稻倒二节的节间直径(RSID)和倒三节的节间直径(RTID)进行QTL分析,共检测到3个控制倒二节茎粗的QTL和7个倒三节茎粗的QTL,分布在第2、3、4、7、11号染色体上。其中2号染色体上的qRSID2-1qRTID2-1距离较近,qRSID2-2/qRTID2-2qRTID2-3可能是同一位点,并在多次试验中被重复检测到,表型贡献率为7.89%~12.36%,物理区间在100 kb之内。

    Abstract:

    The population of G46B/A232 RILs and the high-density genetic linkage map created by resequencing the population were used to map QTLs for internode diameter of reciprocal second internode and reciprocal third internode with the R/qtl software package and CIM method.A total of 3 QTLs for the diameter of reciprocal second internode and 7 QTLs for the diameter of reciprocal third inter nodes were detected,which were distributed on chromosomes 2,3,4,7,and 11.On chromosome 2,qRSID2-1 and qRTID2-1 are relatively close.qRSID2-2/qRTID2-2 and qRTID2-3 repeatedly detected in multiple trials may be the same QTL,with phenotypic contribution rates of 7.89%-12.36% and the physical interval within 100 kb on chromosome 2.The genetic study of the internode diameter of reciprocal second internode and reciprocal third internode in rice will lay a foundation for fine mapping QTLs for the internode diameter,which is beneficial to the molecular breeding and germplasm innovation of rice varieties with lodging resistance and ideal plant type.

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朱小平,李传旭,宁卫东,张建国,曾静,毛毳,李永洪,林拥军.利用重组自交系群体定位水稻茎粗QTL[J].华中农业大学学报,2022,41(4):178-183

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  • 收稿日期:2022-05-12
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  • 在线发布日期: 2022-10-12
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