1.华中农业大学生命科学技术学院/作物遗传改良全国重点实验室，武汉430070;2.农业农村部西南水稻生物学与遗传育种重点实验室，德阳618000
朱小平，E-mail： zhuxiaoping@webmail.hzau.edu.cn
林拥军，E-mail： yongjunlin@mail.hzau.edu.cn
S511.2+1
国家转基因重大专项（2016ZX08001-001）
- ZHU Xiaoping 1
- LI Chuanxu 1
- NING Weidong 1
- ZHANG Jianguo 1
- ZENG Jing 1
- MAO Cui 1
- LI Yonghong 2
- LIN Yongjun 1
1.National Key Laboratory of Crop Genetic Improvement/ College of Biology Science and Technology，Huazhong Agricultural University， Wuhan 430070，China;2.Ministry of Agriculture and Rural Affairs Key Laboratory of Southwest Rice Life and Genetic Breeding，Deyang 618000，China
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为开展水稻茎粗相关基因的精细定位，利用岗46B/A232的高世代重组自交系群体和基于重测序构建的高密度遗传连锁图谱，采用复合区间作图法（CIM），利用R/qtl软件包对水稻倒二节的节间直径（RSID）和倒三节的节间直径（RTID）进行QTL分析，共检测到3个控制倒二节茎粗的QTL和7个倒三节茎粗的QTL，分布在第2、3、4、7、11号染色体上。其中2号染色体上的qRSID2-1和qRTID2-1距离较近，qRSID2-2/qRTID2-2和qRTID2-3可能是同一位点，并在多次试验中被重复检测到，表型贡献率为7.89%~12.36%，物理区间在100 kb之内。
The population of G46B/A232 RILs and the high-density genetic linkage map created by resequencing the population were used to map QTLs for internode diameter of reciprocal second internode and reciprocal third internode with the R/qtl software package and CIM method.A total of 3 QTLs for the diameter of reciprocal second internode and 7 QTLs for the diameter of reciprocal third inter nodes were detected，which were distributed on chromosomes 2，3，4，7，and 11.On chromosome 2，qRSID2-1 and qRTID2-1 are relatively close.qRSID2-2/qRTID2-2 and qRTID2-3 repeatedly detected in multiple trials may be the same QTL，with phenotypic contribution rates of 7.89%-12.36% and the physical interval within 100 kb on chromosome 2.The genetic study of the internode diameter of reciprocal second internode and reciprocal third internode in rice will lay a foundation for fine mapping QTLs for the internode diameter，which is beneficial to the molecular breeding and germplasm innovation of rice varieties with lodging resistance and ideal plant type.
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朱小平,李传旭,宁卫东,张建国,曾静,毛毳,李永洪,林拥军.利用重组自交系群体定位水稻茎粗QTL[J].华中农业大学学报,2022,41(4):178-183
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- 收稿日期:2022-05-12
- 在线发布日期: 2022-10-12
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