首页 > 过刊浏览>2022年第41卷第4期 >151-157. DOI:10.13300/j.cnki.hnlkxb.2022.04.019
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嗅闻佛手柑精油对阿尔兹海默症小鼠的神经行为的影响
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作者:
作者单位:

1.华中农业大学食品科学技术学院/环境食品学教育部重点实验室/ 果蔬加工与品质调控湖北省重点实验室，武汉 430070;2.湖北省畜牧良种场，荆州 434010;3.河南工业大学粮油食品学院，郑州 450001

作者简介:

Email: 1460745718@qq.com

通讯作者:

范刚，E-mail：fangang@mail.hzau.edu.cn

中图分类号:

R285;R277.7

基金项目:

中央高校基本科研业务费专项（2662020SPPY002）；湖北省重点研发计划项目（2020BBA049）


Effects of sniffing bergamot essential oil on neurobehavior in mice with Alzheimer’s disease
Author:
Affiliation:

1.College of Food Science and Technology，Huazhong Agricultural University/ Key Laboratory of Environment Correlative Dietology，Ministry of Education/ Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control，Wuhan 430070，China;2.Hubei Province Rearing Fine Breed Livestock Farm，Jingzhou 434010，China;3.College of Food Science and Technology，Henan University of Technology，Zhengzhou 450001，China

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    摘要:

    为研究嗅闻不同浓度佛手柑精油对D-gal和AlCl3联合诱导的阿尔兹海默症模型小鼠的神经保护作用，选用健康的雄性昆明小鼠，通过腹腔注射D-gal（120 mg/kg）和AlCl3（10 mg/kg）持续49 d以建立阿尔兹海默症实验模型，第29 天起，嗅闻试验组小鼠嗅闻25%或75%佛手柑精油，每天1 h，持续21 d，嗅闻试验结束后评估小鼠行为和大脑生物化学的变化。行为学实验结果显示，嗅闻佛手柑精油显著改善了阿尔兹海默症模型小鼠的认知障碍和探索性下降等症状。生化实验结果显示，嗅闻75%佛手柑精油明显抑制了氧化应激的程度和乙酰胆碱酶活性的增加，促进了小鼠认知能的提高。结果表明，嗅闻75%佛手柑精油可以减轻D-gal和AlCl3引发的小鼠认知行为障碍和生化损伤。

    Abstract:

    Bergamot essential oil （BEO） is known for having many health benefits. Healthy male kunming mice were selected to establish the model mice with Alzheimer’s disease by intraperitoneal injection of D-gal （120 mg/kg） and AlCl3 （10 mg/kg） for 49 days to study the neuroprotective effect of sniffing different concentrations of bergamot essential oil on the model mice with Alzheimer’s disease induced by the combination of D-gal and AlCl3. From the 29th day，the mice in the sniffing group sniffed 25% or 75% bergamot essential oil for 21 days，1 h everyday. After the sniffing experiment，the changes in the behavior and brain biochemistry of the mice were evaluated. The results of behavioral studies showed that sniffing bergamot essential oil significantly improved symptoms including cognitive impairment and exploratory decline in the model mice with Alzheimer’s disease. The results of biochemical studies showed that sniffing 75% bergamot essential oil significantly inhibited the degree of oxidative stress and the increase of acetylcholinesterase （AchE） activity，and promoted the improvement of cognitive function in mice. It is indicated that sniffing 75% bergamot essential oil attenuates D-gal and AlCl3-induced cognitive-behavioral impairment and biochemical damage.

    图1 嗅闻 BEO对 D-gal 和 AlCl3处理的小鼠在OFT 中的水平跨越格子数（A）和直立次数（B）的影响Fig.1 Effects of the inhaled BEO on the number of squares across （A） and the number of uprights（B） of the D-gal and AlCl3 treated mice in the OFT
    图2 嗅闻BEO对D-gal 和 AlCl3处理的小鼠在中自发交替百分比（A）和进入新臂的次数（B）的影响Fig.2 Effects of the inhaled BEO on the percentage of alternation （A） and the number of entries into the new arm （B） of the D-gal and AlCl3 treated mice
    图3 嗅闻 BEO对D-gal和AlCl3处理的小鼠血清中MDA浓度（A）、SOD水平（B）、海马（C）和皮层（D）中GPX水平的影响Fig.3 Effects of the inhaled BEO on the concentration of MDA （A），SOD （B），GPX levels in the hippocampus （C） and cortex （D） of D-gal and AlCl3-treated mice
    图4 嗅闻 BEO对 D-gal 和 AlCl3处理的小鼠的海马（A）和皮层（B）中的AchE水平的影响Fig.4 Effects of the inhaled BEO （25% and 75%） on the AchE level in hippocampus（A） and cortex（B） in the D-gal and AlCl3 treated mice
    图5 小鼠海马组织H&E染色结果（400×）Fig.5 HE （400×） staining results of mice hippocampus in groups
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胡慧艳,文志强,任婧婻,李晓,范刚,潘思轶,张璐璐.嗅闻佛手柑精油对阿尔兹海默症小鼠的神经行为的影响[J].华中农业大学学报,2022,41(4):151-157

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  • 收稿日期:2021-12-16
  • 在线发布日期: 2022-10-12
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