堆肥发酵功能菌株的筛选及其在鸡粪好氧堆肥中的应用
CSTR:
作者:
作者单位:

华中农业大学动物科学技术学院, 武汉 430070

作者简介:

张晟国,E-mail:1144896245@qq.com

通讯作者:

吴健,E-mail:wujian@mail.hzau.edu.cn

中图分类号:

S141.4;X713

基金项目:

国家重点研发计划项目(2018YFD0500204)


Screening of functional strains for composting fermentation and its application in aerobic composting of chicken manure
Author:
Affiliation:

College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    为开发畜禽粪便好氧堆肥的高效降解转化和除臭固氮菌剂,进一步提高堆肥效率和品质,通过菌株产酶和除臭能力分析筛选堆肥发酵功能菌株,分别研制了2种复合菌剂(复合菌剂Ⅰ和复合菌剂Ⅱ),探究添加复合菌剂对鸡粪好氧堆肥常规理化指标、纤维素酶和脲酶活性、氮损失、堆肥产品质量的影响。结果显示:与空白对照组和实验室前期研制的复合菌剂Ⅲ相比,复合菌剂Ⅰ因含有机物质降解能力较高的菌株,能显著增加堆体纤维酶活性,后期纤维素酶酶活高达4.937 U/g;添加复合菌剂Ⅰ可显著提高堆体温度,最高温度可达65.8 ℃,比对照组高5.8 ℃;而复合菌剂Ⅱ因含有除臭能力较强的菌株,能显著降低堆体的脲酶活性和氮损失;添加复合菌剂Ⅱ提高了堆肥产品的总养分含量,堆肥产物总养分可达到5.32%,显著高于对照组(4.52%)。2种复合菌剂均能加快堆肥腐熟,堆肥第7天时,种子发芽指数(GI)值分别为75.12%和75.29%,而对照组GI值仅为47.89%。结果表明,本研究研制的复合菌剂Ⅰ和Ⅱ在鸡粪好氧堆肥应用中有良好的效果,可使堆肥升温、腐熟程度高,增加纤维素酶活性,降低脲酶活性,减少堆肥产物总养分流失和氮损失率。

    Abstract:

    Livestock manure causes serious environmental pollution problems, and aerobic composting can realize the harmless treatment of livestock manure. However, traditional aerobic composting still has problems such as slow temperature rise, long cycle, serious nutrient loss and serious odor volatilization, and the efficiency and quality of composting need to be further improved. The addition of external microbial inoculants can improve the traditional natural composting process of livestock manure. The functional strains for composting fermentation were screened by analyzing the enzyme production and deodorization ability of the strains, and two kinds of compound microbial inoculants were obtained to develop the microbial inoculants with high efficiency of degradation, transformation, deodorization and nitrogen-fixation for aerobic composting of livestock and poultry manure, and to further improve the efficiency and quality of composting. The effects of adding compound microbial inoculants on the conventional physical and chemical indexes, the activity of cellulase and urease, the loss of nitrogen and the product quality of aerobic composting of chicken manure were studied. The results showed that the compound microbial inoculant significantly increased the cellulase activity of the composting due to its higher ability to degrade organic matter, and the activity of cellulase reached 4.937 U/g at the later stage compared with the control and the compound microbial inoculant Ⅲ. Adding compound microbial inoculant Ⅰ significantly increased the temperature of the composting, and the maximum temperature reached 65.8 ℃, which was 5.8 ℃ higher than that of the control. The compound microbial inoculant Ⅱ significantly reduced the activity of urease and the loss of nitrogen of the composting because it contained the bacteria with strong ability of deodorization, and the loss of nitrogen was lower than that of the control. The content of total nutrient and the quality of compost products were improved by adding compound microbial inoculant Ⅱ, and the total nutrient of compost products was 5.32%, which was higher than that of the control(4.52%). The addition of compound microbial inoculant Ⅰ and Ⅱ both accelerated the maturity of compost. On the 7th day of composting, the GI of seed germination index of adding compound microbial inoculant Ⅰ and Ⅱ was 75.12% and 75.29%, while the GI of the control was only 47.89%. It is indicated that the compound microbial inoculants developed has a good effect in the application of aerobic composting of chicken manure, which can increase the temperature of composting and the activity of cellulase, reduce the activity of urease and the loss rate of total nutrient and nitrogen of compost products.

    图1 复合菌剂接种鸡粪堆肥过程中温度(A)、pH(B)、含水率(C)和种子发芽指数(D)的变化Fig.1 Changes of physicochemical indexes including temperature(A),pH(B),moisture(C)and seed germination index (D) during composting of chicken manure inoculated with compound microbial inoculants
    图2 堆肥过程中铵态氮(A)、硝态氮(B)和总氮(C)含量的变化Fig.2 Changes of ammonium nitrogen (A),nitrate nitrogen (B) and total nitrogen (C) content during composting
    图3 添加复合菌剂鸡粪堆肥过程中纤维素酶(A)和脲酶活性(B)变化Fig.3 Changes of cellulase (A) and urease (B) activity during chicken manure composting with compound microbial inoculants
    表 1 功能菌株产淀粉酶、蛋白酶、纤维素酶的透明圈直径和菌落直径之比Table 1 The ratio of transparent circle diameter to colony diameter of amylase,protease and cellulase produced by functional strains
    表 2 堆肥产物理化性质Table 2 Physical and chemical properties of compost products
    参考文献
    相似文献
    引证文献
引用本文

张晟国,彭彦,李茹玉,李展鑫,黄逸豪,王国磊,任竹青,吴健.堆肥发酵功能菌株的筛选及其在鸡粪好氧堆肥中的应用[J].华中农业大学学报,2022,41(4):20-27

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-04-08
  • 在线发布日期: 2022-10-12
文章二维码