基于近红外光谱技术的远安黄茶品质快速无损检测方法
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国家茶叶产业技术体系项目(CARS-19); 中央引导地方科技发展专项(2018ZYYD009); 国家自然科学基金项目(31400586); 湖北省农业科技创新中心项目(2016-620-000-001-032)


Fast and non-destructive quality evaluation of Yuan’an yellow tea based on near-infrared spectroscopy
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    摘要:

    应用近红外光谱(near infrared spectroscopy,NIRS)技术结合多种算法对远安黄茶品质开展快速无损评价。首先通过扫描获得远安黄茶90个样品的近红外光谱,再利用11种不同方法对光谱进行预处理,剔除部分噪声信息,然后应用反向区间偏最小二乘法(backward interval partial least squares,Bi-PLS)筛选反映样品品质的特征光谱区间,应用遗传算法(genetic algorithm,GA)精准提取特征光谱波长,建立了5种黄茶品质预测模型,最后对光谱官能团信息进行解析。结果表明,最佳光谱预处理方法为多元散射校正,Bi-PLS筛选出的特征光谱区间主要为9 003.2~7 497.9 cm-1、6 101.7~5 449.8 cm-1 和4 601.3~4 246.5 cm-1,GA筛选出75个特征光谱波长,建立的Bi-GA-PLS〖JP3〗组合模型具有最佳的稳健性,可准确地预测远安黄茶样品外部品质分数(R2=0.951,RMSEP=1.57,RPD=5.27),初步实现了远安黄茶品质的快速、准确预测。光谱信息解析结果显示,45个光谱波长反映—CHx、C=O和—NHx能团信息,代表单糖、咖啡碱、茶氨酸和游离蛋白质等内含成分物质,30个光谱波长反映O—H、酰胺键以及C—H和C—C伸缩的组合频信息,代表木质素、淀粉、纤维素等多糖内含成分物质。

    Abstract:

    Near infrared spectroscopy (NIRS) combined with a variety of algorithms was used to conduct rapid and non-destructive quality evaluation of Yuan’an yellow tea.The near-infrared spectra of 90 samples of Yuan’an yellow tea were obtained by scanning,and 11 different methods were used to preprocess the spectra to remove part of the noise information.Then the backward interval partial least squares (BiPLS) method was used to screen the characteristic spectral interval reflecting the quality of the sample and the genetic algorithm (GA) was used to accurately extract the characteristic spectral wavelength.Five NIRS prediction models of yellow tea quality were established.Finally,an attempt was made to analyze information of the spectral functional group.The results showed that the best spectral pretreatment method was multiple scattering correction.The characteristic spectral interval screened with BiPLS method was 9 003.2-7 497.9 cm-1,6 101.7-5 449.8 cm-1 and 4 601.3-4 246.5 cm-1,respectively.GA algorithm accurately screened 75 characteristic spectral data points.The Bi-GA-PLS model had the best robustness and predicted the quality of unknown samples (R2=0.951,RMSEP=1.57,RPD=5.27),which was preliminarily realized to quickly and accurately forecast the quality of Yuan’an yellow tea.By analyzing the spectral information,45 spectral data points mainly reflected the information of functional group including —CHx,C=O and —NHx,representing the monosaccharide,caffeine,theanine and free protein closely related to the quality of the samples.The other 30 spectral data points mainly reflected the information of the functional group including O—H,amide bond and C—H and C—C stretching,representing lignin,starch and cellulose.

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王胜鹏,郑鹏程,桂安辉,滕靖,刘盼盼,叶飞,高士伟,马梦君,刘小英.基于近红外光谱技术的远安黄茶品质快速无损检测方法[J].华中农业大学学报,2022,41(1):238-245

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  • 收稿日期:2021-07-21
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  • 在线发布日期: 2022-01-28
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