This article carried out two singlefactor simulation experiments based on computational fluid dynamics software to study the effects of different speeds (60,120,180,240 r/min) on the slurry circulation and the effects of different heating temperatures (45,50,55,60℃) on the temperature increase of the slurry at a certain speed in order to study characteristics of the flow and heat transfer of the slurry in the internal circulation slurry aerobic fermentation reactor,and to further optimize the working parameters of the reactor. To verify the computational model,a bench reactor was built to collect actual data of flow velocity distributed at the outlet of the diversion tube and temperature on the wall of the kettle body. The results showed that when the rotation speed of the stirrer was 120 r/min,the speed range of the nonstirring domain was 0.01-0.27 m/s,and the velocity vector had smaller fluctuations. A rotation speed of 120 r/min had a higher rate of circulation,with a circulation flow rate per revolution of stirrer of 3.77 L/r. When heating temperature of the wall was 55℃,heating at 120 r/min for 5 min increased the average temperature of the slurry from 15.00℃ to 36.98℃,indicating that the heating effect is obvious. The agreement between the measured results and the simulated results was good. The relative standard deviation between the measured value and the simulated value of the flow rate was 14.29%. The relative standard deviation between the measured value and the simulated value of the temperature was 10.78%. It was proved that the computational model is an effective way of describing the complex and dynamic circulation process of the stirring bioreactor. It will provide a theoretical basis for optimizing the working parameters of reactor.