Porcine deltacoronavirus (PDCoV) is a newly emerged enteric coronavirus. In this study,five samples of piglet intestinal contents with diarrhea from two pig farms in Hebei Province were identified to be positive for PDCoV by RT-PCR assay,followed by the virus isolation through inoculation of treated samples on LLC-PK1 cells. The result showed that two PDCoV strains from various pig farms were successfully isolated and named as PDCoV CHN-HeB-A1 and CHN-HeB-B2 strains respectively,which were further identified by indirect immunofluorescence test (IFA),Western blot and electron microscopy assay. The results of IFA and Western blot confirmed that both virus strains can specifically react with monoclonal antibodies against PDCoV M protein. Virus particles with a diameter of 100-150 nm and a typical crown shape were obviously observed under electron microscopy,verifying both virus strains to be PDCoV. The results of whole genome sequencing and genetic evolution analysis showed that both PDCoV strains were homologous to the Chinese strain,with the similarity ranging from 98.7% to 99.1%,which were in the same cluster as the Hong Kong strain.