小麦麦麸阿拉伯木聚糖的体外降脂作用研究
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国家重点研发计划项目(2018YFC1604001)


Effects of wheat bran arabinoxylan on lipid reduction in vitro
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    摘要:

    采用碱提法从小麦麦麸中提取水不溶性阿拉伯木聚糖(waterunextractable arabinoxylan,WUAX)和水溶性阿拉伯木聚糖(waterextractable arabinoxylan,WEAX),探究WUAX和WEAX的胆酸盐结合力和胰脂肪酶抑制活性,以揭示不同结构类型小麦麦麸阿拉伯木聚糖(arabinoxylan,AX)的体外降脂作用。结果显示,WEAX对胆酸盐的结合率低于15%,WUAX对胆酸盐的结合力高于WEAX。WUAX对牛磺胆酸钠和甘氨胆酸钠盐的结合率分别达到52.87%和38.30%;使用Freundlich方程对等温吸附曲线进行拟合,WUAX对牛磺胆酸钠和甘氨胆酸钠盐的1/n分别为0.51和1.30,表明相较于甘氨胆酸钠盐,WUAX对牛磺胆酸钠的结合更加紧密。与胆酸盐结合力相反,WEAX对胰脂肪酶的抑制率显著高于WUAX,WEAX对胰脂肪酶的抑制率随其质量浓度的增加而增加,在质量浓度为30 mg/mL时,最高抑制率为35.93%。另外,反应速率曲线和LineweaverBurk曲线结果表明WEAX对胰脂肪酶的抑制是可逆性抑制,抑制类型是混合型抑制。WUAX和WEAX的体外降脂作用存在差异,这与二者不同的结构特性相关。

    Abstract:

    Alkaline extraction was used to extract waterunextractable arabinoxylan (WUAX) and waterextractable arabinoxylan (WEAX) from wheat bran. The bile salt binding capacity and pancreatic lipase inhibitory activity of WUAX and WEAX were investigated to reveal the effects of wheat bran arabinoxylan (AX) with different types of structure on lipid reduction in vitro. The results showed that the binding rate of WEAX to bile salt was less than 15%,and the binding capacity of WUAX to bile salt was stronger than that of WEAX. The binding rate of WUAX to sodium taurocholate hydrate and sodium glycocholate hydrate was 52.87% and 38.30%,respectively. The Freundlich equation was used to fit the isothermal adsorption curves,and the 1/n of WUAX to sodium taurocholate hydrate and sodium glycocholate hydrate was 0.51 and 1.30,respectively,indicating that compared with sodium glycocholate,WUAX bound to sodium taurocholate more tightly. Contrary to the binding capacity of bile salt,WEAX had a significantly higher inhibition rate on pancreatic lipase than WUAX. The inhibition rate of WEAX on pancreatic lipase increased with the increase of the concentration,with the highest inhibitory rate of 35.93% when the concentration was 30 mg/mL. The results of the reaction rate curve and the LineweaverBurk curve showed that the inhibition of pancreatic lipase by WEAX was reversible,and the type of inhibition was mixed inhibition. In summary,there are difference in the effect of WUAX and WEAX on lipid reduction in vitro,which is related to the difference in the characteristics of structure. It will provide practical reference and theoretical support for further studying the mechanism of lipid metabolism regulated by AX.

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张修威,袁婷婷,牛猛,张宾佳,贾才华,许燕,赵思明.小麦麦麸阿拉伯木聚糖的体外降脂作用研究[J].华中农业大学学报,2021,40(5):

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  • 收稿日期:2021-05-12
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  • 在线发布日期: 2021-09-29
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