Abstract:Field experiment was conducted to study the effects of soil disinfection by dazomet on the control efficacy of cabbage clubroot disease and soil fungal communities analyzed by high through-put sequencing of ribosomal genes in the high mountain area of Lichuan city of Hubei Province.Results showed that the control efficacy of soil disinfection by dazomet (DA) was 91.56%,significantly higher than that of fluazinam (FL) and control (CK).Compared with FL and CK,the yield of DA was increased 63.18% and 237.33%.The net income of DA was increased 19.38% and 93.81%.The result of quantitative real-time PCR showed that there were 415 spores/g in the soil by DA treatment,lower than that by FL (3 120 spores/g) and significantly lower than that in CK (12 833 spores/g) (P<0.05).The result of high through-put sequencing of ribosomal genes showed that there were 1 842 fungal OTUs (operational taxonomic unit),of which 180 (9.88%) were commonly detected in three treatments.The community structures of fungi in three treatments were similar,but a part of genera had greater changes of abundance.Both DA and FL had a great influence on fungal α diversities.Among 4 indexes,the Chao1 and ACE indexes of DA and FL were significantly lower than that of CK (P<0.05).The result of analyzing fungal β diversities showed that DA and FL were close to each other in distance,but further away from CK.The result of Metastats analyses showed that under DA and FL treatments,there were 15 and 29 genera with significant changes of abundance,respectively.The above results demonstrated that soil disinfection by DA can effectively control cabbage clubroot with significantly higher economic profits,while had no significant impact on soil fungal diversity.