The isoenzymes of polyphenol oxidase from Camellia sinensis var. Longjing 43 fresh leaves were isolated and purified with ammonium sulfate precipitation,ion exchange chromatography and gel filtration chromatography.One single isoenzyme (PPO Ⅴ) was purified.Its subunit size was about 80 ku.It will be useful for further studying the properties of tea PPO isozymes.