Gene expression profile of Petunia hybrida was used to screen cold responsive gene,then one CCCH-type zinc-finger protein gene PhTZF1 was identified.Its corresponding full length cDNA in Petunia hybrida was amplified via RT-PCR.The full length of the coding region was 2 085 bp，putatively encoding 694 amino acid residuals,and contained 2 CCCH-type zinc-finger domains.The analyses of phylogenic tree showed that the PhTZF1 was similar to the AtSZF1 in Arabidopsis thaliana.Through analyzing expression profile of tissues with Semi-Quantitative RT-PCR,the PhTZF1 was found to express in root,stem,leaf and flower,but with very low level.The results of real-time PCR showed that PhTZF1 gene was up-regulated in different degrees under cold,drought,ABA,MeJA,salt and hypertonic stresses,while it was most sensitive to cold stress,indicating that the PhTZF1 gene was related with the responses to cold,drought and other abiotic stresses.