Distributions of both male cones and female cones in a tree crown and the ratio of female-male gamete were studied in Pinus massoniana clone seed orchard located in Taizishan,Hubei Province.The results showed that there were significant differences among the quantity of both male cones and female cones in the tree crown.The average number of male cones was 1 009.09,ranging from 291.61 to 1 549.69 per tree whereas the average number of female cones was 343.91,ranging from 135.43 to 636.39 per tree.The ratio of male cones to female cones was ranged from 0.14 to 1.07 for different clone mother trees.The average pollen number per sac,the average pollen per tree and all pollen in seed orchard was 16 513.33,3.75×1011 and 2.03×1015,respectively.There was a significant difference for pollen yield among different clone trees.The average ovule number per female cone,average ovule number per tree and all ovule in seed orchard was 177,60 872.07 and 3.29 billion,respectively.The ratio of pollen to ovule for the seed orchard of P.massoniana studied was about 1 to 6.16×106.The seed yield in this seed orchard could theoretically reach to 4 115.53 kg.