To define the difference of the resistance level of asparagus stem blight fungus Phomopsis asparagi (Sacc.) Bubak from different provinces on systemic and protective fungicides,the resistance levels of 24 isolates from 6 provinces on carbendazim and mancozeb were determined by using the mycelial growth rate method. The isolates from Shandong Province expressed the most resistance on carbendazim with an average EC50 value of 1.10 mg/L, followed by the isolates from Jiangxi Province and Fujian Province, with an average EC50 values of 0.58 mg/L and 0.33 mg/L respectively. The isolates from Fujian Province expressed the most resistance on mancozeb with an average EC50 value of 34.53 mg/L,followed by the isolates from Hebei Province and Shandong Province, with average EC50 values of 15.88 mg/L and 14.19 mg/L respectively. Compared to mancozeb,it's easier for carbendazim to produce resistance,with 7 resistant isolates,3.66 as the resistant level and 29.17% as the resistant frequency,while 4 resistant isolates,with 1.90 as the resistant level and 16.67% as the resistant frequency, were included in mancozeb. Of 24 isolates,SD4 revealed resistance on both of carbendazim and mancozeb,with the resistance levels of 10.44 and 3.01 respectively,which expressed multiresistance.