The enzyme activities,distribution features and changing patterns with feeding time of pepsin,trypsin,lipase and amylase in digestive organs for pond-cultured paddlefish were determined in the present study.The results showed that the highest activity of pepsin was found in the oesophagus,then followed in order by the liver,stomach,pyloric caeca,and intestine tract.The duodenum had the highest activity of trypsin and lipase,then followed in order by the liver,pyloric caeca,stomach,and oesophagus.The activities of amylase from the highest to the lowest in the digestive organs was in the order of the duodenum,oesophagus,stomach,liver,and pyloric caeca.During the culturing period from June to October,the activity of pepsin decreased at first,and then increased,with the lowest value appeared in August.The activities of trypsin increased gradually with increasing of the feeding time.While the activities of lipase and amylase increased at first and then declined,with the highest value appeared in September.