Factors affecting plant regenerations of four rose cultivars ( Rosa multiflora Thunb.) were studied with leaves harvested directly from fields to further determine the optional culture condition and high frequent regeneration genotypes.Results showed that there was significant difference among the adventitious bud regeneration. The highest one (78.20%) was from R.multiflora 2,the second (61.16%) from R.multiflora 3,the third (13.00%) from R.multiflora 4 and the fourth (5.93%) from R.multiflora .The regeneration rate of leaves harvested directly from field is higher than that of leaves from sterile seedling in vitro culture.The results indicted that genotypes,plant hormones and the source of the explants had significant effect on the adventitious shoots induction.It is important to select suitable genotype and optional condition in vitro tissue culture of R.multiflora species.