Artificial gynogenesis has been proved to be a valuable genetic tool for the rapid production of inbred lines,development of all female populations and construction of linkage map.In the present study,meiogynogenesis of blunt snout bream (Megalobrama amblycephala) was induced by cold-shock with the eggs being activated by UV genetic inactivated sperms of Cyprinus carpio Songpu carp.DNA contents of gynogenetic individuals were measured by flow cytometry and there were no significant differences between gynogenetic individuals and M.amblycephala normal diploids (P>0.05),indicating that the gynogenetic individuals were diploids.Eight pairs of microsatellite primers could amplify clear parental bands were selected to test the gynogenetic progenies.The results of primers MFW1,MFW5 and HLJ393 revealed that no male parent gene exist in gynogenetic individuals.Then ten microsatellites locus where female parent was heterozygous were used to analyze the homozygous ratio of the induced gynogenetic diploids.The results of genetic analysis showed that a high percentage of heterozygosity was found in gynogenetic diploids except for loci TTF-EST46，TTF-EST61 and TTF-EST851.The rate of heterozygosity in meiogynogenetic progenies at the locus TTF-EST12 was 0.864 and the other six loci were 1.000.The average recombination rate of these 10 loci was 0.686 4.This study confirmed that it was difficult to get high rate homozygotic M.amblycephala gynogenesis through suppression of the meiosis-II,because there was high heterozygosity in the first meiogynogenetic generation owing to crossover between the homologous chromosome during the meiosis-I stage.These gynogenetic diploids could be used as breeding material for its high genetic homogeneity with the female parent.