Abstract:A new method for eradicating the double infected pathogen of CTV and CTLV incitrus was explored in this experiment.The citrus plants were selected from those conserved in net greenhouse of National Indoor Conservation Center of Virus-free Germplasms of Fruit Crops,Huazhong Agricultural University.The infection of pathogen was detected with reverse transcripts PCR (RT-PCR).Results showed that the suitable protocol was using the treatment of the antiviral medicament with Chinese medicines (eight times) and combinated with heat treatment of the highest temperature at 40℃ during the day(6 h/d),the temperature at 25℃ in the evening kept for fifty days in the PC made greenhouse.The protocol mentioned above was proved to completely eradicate double infected pathogen of CTV and CTLV; Furthermore,the combined treatment of shoot-tip grafting and the antiviral Chinese medicines was identified 85.7% eradication of the double pathogen.In above experiments,the leaves were sampled at 1,3,5,7,9 d after the Chinese medicines treatment.The results showed that the activity of the resistance related enzymes PPO,POD,SOD were increased compared with the control.