To obtain the suitable culture system of bulblets growth and enlargement,the effects of different culture systems with appending sucrose,AC,Me-JA to the MS medium on the growth and enlargement of Lilium lancifolium bulblets in vitro were studied.The results showed that bulblets are significantly heavy in the liquid culture system compared with solid and solid-liquid.In the liquid culture system,L.lancifolium can culture bulblets with fresh mass of 249.84 mg and percentages of bulblets (diameter＞8 mm) of 54.05% on the average.The concentration of 90.0 g/L sucrose and 3.0 g/L AC was optimal to enlarge bulblets in vitro with the average fresh mass of 479.17 mg and the biggest of 870 mg.The concentration of 10-7 mol/LMe-JA are most favorable for enlargement of bulblets with the average fresh weight of 180 mg.The comparison trials of planting using bulblets of different diameters indicated that the survive of big bulblet were easier than that of small bulblet.