国家民委科研基金(05ZN011)和中南民族大学自然科学基金项目（YZY09002，YZZ10002）资助
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以地高辛标记的栽培稻基因组（基因组为AA）DNA为探针，对非洲野生稻(基因组为BBCC)的体细胞染色体进行荧光原位杂交分析，研究AA 染色体组和BBCC 染色体组之间的关系，同时对杂交后的染色体进行同源染色体配对。结果表明：栽培稻A基因组和非洲野生稻基因组有较高的同源性，其中高度重复DNA序列在栽培稻和非洲野生稻间具有保守性。
Genomic in situ hybridization（GISH）analysis on Oryza schweinfurthian with digoxigenin-labelled genomic DNA from O.sativa was used to study the relationship between the A genome of O.sativa and BC genome of O.schweinfurthian.Karyotype analysis was made based on the similar band patterns of the hybridization signal.The result showed that A genome of cultivated rice had higher homology with genome of African wild rice,including repetitive DNA sequence conserved between cultivated rice and African wild rice.
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邱小芬,刘 虹,覃 瑞,陈 雁.利用栽培稻基因组DNA对非洲野生稻进行染色体荧光原位杂交分析[J].华中农业大学学报,2010,29(5):537-540
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- 收稿日期:2010-02-11
- 最后修改日期:2010-07-05
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