Ampelopsis grossedentata is a kind of medical0edible plant with many medical and health care functions.In order to explore the effects of selenium on growing development,physiological-biochemical characteristic of A.grossedentata,Na2SeO3 was sprayed on the leaf of potted A.grossedentata with different concentration ( 0，50，75，100，125，150 and 175 mg/L ).The effects of selenium on the content of selenium and the activity of antioxidase,the dynamic variation of soluble protein,free amino acid,soluble sugar and flavone in A.grossedentata were studied.The results showed that when the concentration of selenium was lower than 100 mg/L，the higher the concentration is,the higher the selenium content was.When the concentration of selenium was ranged from 100 to 175 mg/L,the content of selenium in A.grossedentata has no significant change.The activity of GSH-Px could be promoted by selenium,and reached to maximum when the concentration of selenium was 125 mg/L.The activitiy of APX and CAT could be promoted when the concentration of selenium was lower than 125 mg/L.However,the activity of SOD could not be promoted in low concentration of selenium.When the concentration of selenium was ranged from 75 to 125 mg/L,the content of soluble protein,free amino acid,soluble sugar and flavone in A.grossedentata were increased.The activity of antioxidase and the synthesis of effective ingredient in A.grossedentata can be promoted with suitable concentration of selenium,and the optimum spraying concentration of selenium is 100 mg/L.