萝卜-芥蓝异源四倍体F4和F10世代DNA甲基化变异的MSAP分析
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教育部留学回国人员基金项目(4002-082017)资助


Methylation Variation of Different Generations of Artificial Raphanus sativus×Brassica alboglabra Allopolyploids Analysed with MSAP
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    摘要:

    以人工合成的萝卜-芥蓝异源四倍体的F4和F10世代为材料,用MSAP法检测2种材料DNA甲基化变异情况,以期为远缘杂交后不同世代间基因组DNA甲基化遗传和变异规律提供实验依据。结果表明:F4代和F10代甲基化程度分别为30.61%和33.10%,共检测12种甲基化变异模式,可以分为5种类型,甲基化变异位点占所有甲基化位点的41.71%。用甲基化抑制剂5-氮胞苷处理F4代,F10代材料,检测到甲基化程度分别为20.67%和25.75%,F10代比F4代受到了更大冲击,说明不稳定世代对环境的影响更加敏感。

    Abstract:

    Methylation variations of artificial F4 and F10 generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP.The results showed that the methylation levels of F4,F10 were 30.61% and 33.10%,respectively.Twelve different models of methylation variations were detected,and these models could be divided into 5 types,with 41.71% variable methylation sites.The methylation levels of F4 and F10 after 5-azacytidine treatment were 20.67% and 25.75%,respectively.The results showed that after 5-azacytidine treatment,methylation variations of F10 were stronger than those of F4.

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李象松,魏丽华,李炫丽,汪艳杰,王 冰,吴江生,龙 鸿.萝卜-芥蓝异源四倍体F4和F10世代DNA甲基化变异的MSAP分析[J].华中农业大学学报,2010,29(1):96-100

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  • 收稿日期:2009-04-20
  • 最后修改日期:2009-07-15
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