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以人工合成的萝卜-芥蓝异源四倍体的F4和F10世代为材料，用MSAP法检测2种材料DNA甲基化变异情况，以期为远缘杂交后不同世代间基因组DNA甲基化遗传和变异规律提供实验依据。结果表明：F4代和F10代甲基化程度分别为30.61%和33.10%，共检测12种甲基化变异模式，可以分为5种类型，甲基化变异位点占所有甲基化位点的41.71%。用甲基化抑制剂5-氮胞苷处理F4代，F10代材料，检测到甲基化程度分别为20.67%和25.75%，F10代比F4代受到了更大冲击，说明不稳定世代对环境的影响更加敏感。
Methylation variations of artificial F4 and F10 generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP.The results showed that the methylation levels of F4,F10 were 30.61% and 33.10%,respectively.Twelve different models of methylation variations were detected,and these models could be divided into 5 types,with 41.71% variable methylation sites.The methylation levels of F4 and F10 after 5-azacytidine treatment were 20.67% and 25.75%,respectively.The results showed that after 5-azacytidine treatment,methylation variations of F10 were stronger than those of F4.
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李象松,魏丽华,李炫丽,汪艳杰,王 冰,吴江生,龙 鸿.萝卜-芥蓝异源四倍体F4和F10世代DNA甲基化变异的MSAP分析[J].华中农业大学学报,2010,29(1):96-100
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- 收稿日期:2009-04-20
- 最后修改日期:2009-07-15
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