传染性法氏囊病病毒上海超强毒株VP2-4-3基因真核表达质粒的构建与表达
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S852.659.4 S858.31
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上海市科学技术发展基金项目(01JC14034)资助
Construction and Expression of Eukaryotic Expression Plamid of VP2-4-3 Gene of Very Virulent Infectious Bursal Disease Virus Strain SH95
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摘要:
应用Long and Accurate—PPCR(LA—PCR)技术。扩增克隆了鸡传染性法氏囊病病毒上海超强毒(vvIB—DV)的VP2—4—3基因。应用粘性末端连接策略，将VP2—4—3基因克隆人真核表达载体pALTER—MAX。经酶切鉴定，表明VP2—4—3基因为正向插入，位于CMV启动子下游。该真核表达质粒在脂质体的介导下转染Vero细胞，经特异性抗IBDV抗体的免疫荧光检测，发现在细胞内有特异蛋白表达。
Abstract:
VP 2-4-3 gene of very virulent infectious bursal disease virus strain SH95 (vvIBDV) was applied by Long and Accurate-PCR(LA-PCR). Then VP2-4-3 gene was ligated into eukaryotic expression vector pALTER-MAX by cohesive ends. It was proved by enzyme-digestio
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蒋静 孙建和 陆苹.传染性法氏囊病病毒上海超强毒株VP2-4-3基因真核表达质粒的构建与表达[J].华中农业大学学报,2004,23(2):
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