Page 94 - 《华中农业大学学报（自然科学版）》2024年第1期

88 华 中 农 业 大 学 学 报 第 43 卷

Effect of drought stress on composition and structure of
endosymbiotic bacteria in potato leaves

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JI Ze ,XIAO Guanli ,CHEN Bin ,LI Junyi ,YAO Yao ,ZHU Chunyue ,YANG Jinrui 1
1.College of Agronomy and Biotechnology，Yunnan Agricultural University，Kunming 650201，China；
2.College of Plant Protection，Yunnan Agricultural University，Kunming 650201，China.
Abstract High-throughput sequencing of 16S rRNA from endosymbiotic bacteria in potato leaves un⁃
der drought stress was performed with Illumina Novaseq sequencing technology to understand the changes
in the composition and structure of endosymbiotic bacteria in potatoes under drought stress. The effects of
drought stress on the composition， structure， and diversity of endosymbiotic bacteria in potato leaves were
studied. The results showed that 1 548 and 2 006 taxonomic sequences were obtained from potato leaves
under normal watering and drought stress， respectively. Among them， 287 were unique OTUs under nor⁃
mal watering and 769 were unique OTUs under drought stress. Under drought stress， the abundance of the
endosymbiotic bacteria candidatus Portiera and Arsenophonus decreased by 95.9% and 68.1% compared
with that under normal watering， while the abundance of Muribaculaceae， Lachnospira， Lactobacillus，
Lachnospiraceae_NK4A136_group， Rhizobium， Helicobacter， Streptococcus and Desulfovibrio increased
by 203.8%，152.4%，81.8%，89.7%，285.6%，1 428.0%，112.0% and 148.4% respectively under drought
stress compared with that under normal watering. The Shannon index and Simpson index under drought
stress were significantly higher than that under normal watering， while the ACE index and Chao1 index un⁃
der drought stress were not significantly different from that under normal watering. It is indicated that
drought stress can affect the composition of endosymbiotic bacterial communities in potatoes. Under
drought stress， the endosymbiotic dominant bacteria Actinomycetota and Bacillota were enriched， and the
relative abundance of Burkholderiaceae bacteria increased， which may be the positive response of potato to
drought stress.
Keywords potato；endosymbiotic bacteria；drought stress；microbial community；high throughput se⁃
quencing
（责任编辑：张志钰）