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第 1 期 王贵 等：基于 IRAP 标记的沙子空心李遗传多样性评价及指纹图谱构建 11

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and REMAP in eggplant［J］. Acta horticulturae sinica，2008，35 nese with English abstract）.

Evaluation of genetic diversity and construction of DNA fingerprint of

Prunus salicina Lindl. ‘Shazikongxinli’ based on IRAP markers

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WANG Gui ,LI Ruirui ,WU Maohong ,REN Feihong ,WANG Lili ,QIAO Guang 1
1.The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in
Mountain Region（Ministry of Education）/College of Life Sciences，
Guizhou University，Guiyang 550025，China；
2.Tongren Academy of Forestry，Tongren 554300，China；
3.Tongren Academy of Agricultural Sciences，Tongren 554300，China；
4.Guizhou Academy of Agricultural Sciences，Guiyang 550006，China

Abstract Ninty-two accessions of ‘Shazikongxinli’ germplasm with elite traits were selected by visit‐
ing and surveying to evaluate the genetic diversity of Prunus salicina Lindl. ‘Shazikongxinli’ IRAP primers
were developed based on the conserved regions of RT sequences of plum reverse transcription transposons
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Ty1-copia and Ty3-gypsy. L 16 （4 ） orthogonal experiments were designed to optimize the main factors af‐
fecting content of IRAP-PCR with 10 μL volume. 18 IRAP primers were used to analyze the genetic diver‐
sity and construct the fingerprint of the germplasm tested. The results showed that the optimized 10 μL
IRAP-PCR reaction system was 1.3 μL of 5-10 mol/L IRAP primers， 5.0 μL of PCR mixture， 1.0 μL of
template DNA（30 ng/μL）， and the number of PCR cycles was 40 times. A total of 189 loci were ampli‐
fied from 18 IRAP primers with good repeatability and high polymorphism， including 180 polymorphic loci.
The average value of observed number of alleles（N a ）， effective number of alleles（N e ）， Nei’s gene diversi‐
ty（H e ） and Shannon’s information index（I） for all primers was 1.930，1.426，0.261 and 0.405， respective‐
ly. The 92 accessions tested were clustered into 4 groups using the neighbor-joining method. The IRAP
primers Ty3-2 and Ty3-6 were identified as core primers， which can be used to efficiently construct the fin‐
gerprints of 92 accessions tested.
Keywords Prunus salicina Lindl. ‘Shazikongxinli’；germplasm； molecular marke； IRAP； finger‐
printing； genetic diversity； germplasm innovation

（责任编辑：张志钰）