Page 47 - 《华中农业大学学报（自然科学版）》2022年第6期

第 6 期 徐慧芳 等：不同耕作模式对稻田土壤真菌丰度及群落结构的影响 41

2484.WANG N，PAN X C，WANG C K，et al.Effects of simu‐ 804-810（in Chinese with English abstract）.
lated acid rain on soil fungi diversity in the transition zone of mo‐ ［23］ DEACON L J，JANIE PRYCE-MILLER E，FRANKLAND J
so bamboo and broadleaf forest［J］.Environmental science，2020， C，et al.Diversity and function of decomposer fungi from a grass‐
41（5）：2476-2484（in Chinese with English abstract）. land soil［J］.Soil biology and biochemistry，2006，38（1）：7-20.
［20］ ZHANG X F，ZHAO L，XU S J，et al.Soil moisture effect on ［24］ 张立成，邵继海，林毅青，等 . 稻-稻-油菜轮作对土壤微生物活
bacterial and fungal community in Beilu River（Tibetan Plateau） 性和多样性的影响［J］. 生态环境学报，2017，26（2）：204-210.
permafrost soils with different vegetation types［J］.Journal of ap‐ ZHANG L C，SHAO J H，LIN Y Q，et al.Influence of microbial
plied microbiology，2013，114（4）：1054-1065. diversity and activity of soil on the rice-rice-rape rotation［J］.
［21］ ROMANÍ A M，FISCHER H，MILLE-LINDBLOM C，et al. Ecology and environmental sciences，2017，26（2）：204-210（in
Interactions of bacteria and fungi on decomposing litter：differen‐ Chinese with English abstract）.
tial extracellular enzyme activities［J］. Ecology，2006，87（10）： ［25］ DE VRIES F T，GRIFFITHS R I，BAILEY M，et al.Soil bacte‐
2559-2569. rial networks are less stable under drought than fungal networks
［22］ 张慧，马连杰，杭晓宁，等 . 不同轮作模式下稻田土壤细菌和真 ［J/OL］. Nature communications，2018，9：3033［2022-03-24］.
菌 多 样 性 变 化［J］. 江 苏 农 业 学 报 ，2018，34（4）：804-810. https：//doi.org/10.1038/s41467-018-05516-7.
ZHANG H，MA L J，HANG X N，et al.Changes of soil bacterial ［26］ MANZONI S，SCHIMEL J P，PORPORATO A.Responses of
and fungal diversity in paddy soils under different rotation pat‐ soil microbial communities to water stress：results from a meta-
terns［J］. Jiangsu journal of agricultural sciences，2018，34（4）： analysis［J］.Ecology，2012，93（4）：930-938.

Effects of farming patterns on abundance and community structure of

fungi in paddy soils

2
1
1
2
2
XU Huifang ,WAN Ziwei ,SHENG Rong ,WEI Wenxue ,ZHANG Wenzhao ,HUANG Guoqin 1
1.Center for Ecological Sciences，Jiangxi Agricultural University，Nanchang 330045，China；
2.Taoyuan Station of Agro-Ecology Research，Institute of Subtropical Agriculture，Chinese Academy of Sci⁃
ences/Key Laboratory of Agro-Ecological Processes in Subtropical Regions，Changsha 410125，China
Abstract Four farming patterns including double rice（DR），middle rice（MR），middle rice-rape‐

seed rotation（MROR），and middle rice-pakchoi-rapeseed rotation（MRPOR）in a long-term（10 years）
practices were selected to analyze the abundance and differences in community structure of fungi under dif‐
ferent farming systems in China. The abundance and community composition of fungi in paddy soils were
determined by real-time fluorescence quantitative PCR and high-throughput sequencing to analyze the differ‐
ences in the abundance and community structure of fungi in soil under different farming patterns and their in‐
fluencing factors. Results showed that MR，MROR and MRPOR had a significant influence on the abun‐
dance of fungi. MRPOR increased the abundance of fungi. The result of correlation analyses showed that ni‐
trate nitrogen was significantly and positively correlated with the abundance of fungi. The crop rotation had
a significant influence on the gene community structure of fungi in soil. The result of redundancy analyses
showed that the content of soil water was the most significant factor affecting the community structure of
fungi. In conclusion，the abundance，composition and diversity of communities of fungi in soil were differ‐
ent under different farming patterns of rice. The abundance of fungi was greatly affected by the content of
nitrate nitrogen in soil under different farming patterns，and the community structure of fungi was mainly af‐
fected by the content of water in soil.
Keywords paddy field；soils；farming patterns；fungi；abundance；community structure；paddy-up‐
land rotation field

（责任编辑：张志钰，赵琳琳）