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42 华 中 农 业 大 学 学 报 第 41 卷

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Effect of adding high-temperature cellulose-degrading bacteria on
nitrogen loss during high-temperature composting

SHAN Yajing,WU Kuntan,GAO Jianwei,YANG Xuchen,ZHENG Rong
College of Animal Science and Technology，Huazhong Agricultural University，Wuhan 430070，China

Abstract In order to explore the application effect of cellulose-degrading bacteria in compost decom‐

posing，the experiment mixed the carcasses of livestock and poultry with straw after high temperature treat‐
ment，and divided them into the control group without adding bacterial agent and adding 1% high tempera‐
ture resistant cellulose bacteria（Parageobacillus thermoglucosidasius）.The compost was composted by aer‐
obic static ventilation for 28 days，and the physical and chemical parameters（temperature，pH，moisture
content，ammonium nitrogen，nitrate nitrogen，nitrite nitrogen，total carbon and total nitrogen）and nitrogen
conversion function during the composting process were determined.The results showed that the ammoni‐
um nitrogen content and nitrate nitrogen content of the bacterial agent group were significantly higher
（P<0.05）than the control group，but the final total nitrogen content of the bacterial agent group was lower
than the control group.The copy number of nirK in the control group in the pre-composting period was sig‐
nificantly higher than that in the inoculum group（P<0.05）and was significantly positively correlated with
+
the content of NH 4 （P<0.05）；the copy number of nirS in the inoculum group was extremely significant‐
-
ly higher than that in the control group（P<0.01），and has a very significant positive correlation with NO 3
content（P<0.01）.In the middle stage of composting，the copy number of narG in the control group was
+
significantly higher than that in the inoculum group（P<0.01），and the correlation with NH 4 content was
close to the significant level（P=0.064）；in the late stage of composting，the copy number of nosZ in the
control group was extremely significantly higher than bacterial agent group（P<0.01）.The high tempera‐
ture-resistant cellulose-degrading bacteria mainly change the nitrogen cycle of the compost through ammo‐
nia oxidation and denitrification in the early stage of composting，but also promotes denitrification and in‐
creases nitrogen loss.
Keywords livestock and poultry carcasses；high-temperature treatment；composting；nitrogen loss；

cellulose-degrading bacteria

（责任编辑：边书京）