Page 52 - 《华中农业大学学报（自然科学版）》2022年第2期

第 2 期 王友强 等：硼氮互作对甘蓝型油菜生长、氮素吸收和籽粒产量的影响 47

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Effects of boron-nitrogen interaction on growth，nitrogen uptake and

seed yield of rapeseed（Brassica napus）

1 1 1 1 2 1
WANG Youqiang ,ZHAO Zhe ,WANG Sheliang ,YE Xiangsheng ,LIU Qingyun ,XU Fangsen
1.Microelement Research Center/College of Resources and Environment，
Huazhong Agricultural University，Wuhan 430070，China；
2.Xishui Agricultural Technology Extension Center，Huanggang 438000，China

Abstract To explore the yield⁃increasing effect and physiological mechanism of boron (B)⁃nitrogen

(N) interaction in Brassica napus, the effects of B⁃N different treatments on rapeseed growth, N uptake and
utilization, N metabolism enzyme activity, and seed yield were analyzed by hydroponic culture experiment
and field plot experiment. The results showed that B deficiency significantly inhibited the root growth of rape⁃
seed at the seedling stage under different N supply levels. Compared with low N or low B treatment, the bal⁃
anced supply of B and N significantly increased the N uptake and the expression level of related genes, and
improved the activities of nitrate reductase (NR) and glutamine synthetase (GS) in N metabolism. In the field
experiment, the application of B and N fertilizer significantly increased the seed yield of rapeseed. However,
the effect of B fertilizer application was closely related to soil N availability. Under the normal N supply level
2
2
(180 kg/hm ), the effect of B fertilizer application was better than that of low N (60 kg/hm ) condition,
2
2
while high B (18 kg/hm ) combined with high N (240 kg/hm ) limited the further increase of seed yield. Ap⁃
propriate co⁃application of B and N significantly improved the N harvest index and promoted the distribution
of N to the grains. B and N co⁃application had significant interaction effects on the growth and development
of rapeseed, both in the hydroponic seedling stage and in the field of the whole growth stage. Under the field
2
conditions of this study, the recommended amount of N fertilizer was 180⁃240 kg/hm , and the combined ap⁃
2
plication with 4.5⁃9 kg/hm of borax could maximize the yield increasing potential of rapeseed.
Keywords Brassica napus；boron and nitrogen interaction effect；precise fertilization；nitrogen metab⁃
olism enzyme activity；comprehensive management of nutrients；balanced fertilization；green and efficient

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