Page 42 - 《华农农业大学学报》2020年第3期

第 ３ 期 张明菊 等:黄萎病对海 / 陆嫁接棉苗叶片中 H ２O ２ 、 抗病相关酶活性和 GST 、 PAL 基因表达的影响 ３ ７

EffectsofVerticilliumdahliaeonH ２ O ２contentactivitiesofdisease
,
resisitanceＧrelatedenz y mesg eneex p ressionofGSTandPAL
,
inleavesofu p landseaislandcottong raftedseedlin g s
/

ZHANG Min gj u , ZHANG Hao , ZHU Hua g uo , XIA Qizhon g

Colle g eo fBiolo gyandA g riculturalResources / HubeiProvinceKe yLaborator yo fGerm p lasm
Im p rovementandCom p rehensiveUtilizationo fEconomicForestTreeResources ,
Huan gg an g NormalUniversit y Huan gg an g４３８０００ , China
,

Abstract Goss yp iumbarbadense / G．hirsutum g raftedcottonseedlin g s ( S / R ) wereobtainedb yuＧ
sin gG．hirsutum cottonPima９０ ( R ) resistanttoVerticillium wiltastherootstockandG．barbadense
cottonvariet yEmian２２ ( S ) susce p tibletoVerticillium wiltinHubeiProvinceasscion．ThediseasereＧ
sistanceafterincubationofPima９０ ( R ), Emian２２ ( S ) andg raftedcottonseedlin g s ( S / R ) wasstudied．
H ２O ２contentinleaveswasdetermined．ThedifferencesinthelevelsofPOD / PALenz y meactivit yand
theex p ressionp atternsofPAL / GST g eneswerecom p ared．Resultsshowedthatwiltin gde g reeofthe
G．barbadense / G．hirsutum g raftedcotton ( S / R ) aftertheincubationofdama g edcottonrootwith
p atho g ensus p ensionswasbetweenthatoftherootstockPima９０ ( R ) andscionEmian２２ ( S ), indicatin g
that g raftin g im p rovedtheresistanceofcottonseedlin g stoVerticillium wilt．H ２O ２contentsinleaves
andtheenz y maticactivitiesofPODandPALinEmian２２ ( S ), Pima９０ ( R ) andthe g raftedcotton ( S / R )
wereobviousl yincreasedafterincubation withVerticillium dahliae Kleb．However , H ２O ２ contentin

leaves , PODandPALactivitiesincreasedslowl y inEmian２２ ( S ), ra p idl y inPima９０ ( R ), andmoderatel y
intheS / Rg raftedcotton．Pima９０ ( R ) andtheg raftedcotton ( S / R ) showedsimilartendenc yofchanＧ
g in g ．ResultsofrealＧtimePCRshowedthatthemRNAex p ressionlevelofGST g eneinthreecottonsinＧ
creasedfirstl yandthendecreased．ThedurationofGST g eneex p ressionforEmian２２ ( S ), Pima９０ ( R )

andg raftedcotton ( S / R ) was８Ｇ１６hand８Ｇ４８h , res p ectivel y ．Thep atternsofPAL g eneex p ressionin
,
thethreecottonsweresimilar．Ex p ressionlevelofPAL g enewasincreasedfirstl ythendecreased , and
,
increasedsubse q uentl y withtwice p eakat８hand４８h．The p eakvalueinEmian２２ ( S ), Pima９０ ( R ) and
g raftedcotton ( S / R ) waslow , hi g h , andmoderate．
Ke y words Goss yp ium s pp ． ; Goss yp ium barbadense / G．hirsutum g raftedcotton ; Verticillium
;
dahliae Kleb ; H ２O ２ diseaseresisitanceＧrelatedenz y mes ; GST andPAL g enes

( 责任编辑: 张志钰)