The field in situ control experiments with 3 treatments including removing current year’s litter （NL）， natural state （CK）， and doubling current year’s litter （DL） were conducted to study the response characteristics of fungal communities in soil of Calamagrostis angustifolia wetlands in Sanjiang Plain to the treatments of litter.The effects of the treatments of litter on the structure， diversity， functional taxa and key driving factors of fungal community in soils by combining high-throughput sequencing technology with the function prediction platform of FUNGuild.The results showed that the treatments of litter significantly affected the alpha-diversity of fungi in soil.Compared with CK， NL significantly reduced the ACE and Shannon index of fungi， DL significantly increased the ACE and Shannon index of fungi（P<0.05）.Ascomycota and Basidiomycetes were the dominant phyla， with both NL and DL treatments increasing the relative abundance of Ascomycota and decreasing the relative abundance of Basidiomycota（P<0.05）.There were significant differences in the relative abundance of functional taxa in fungi among different treatments.Compared with CK， NL significantly increased the number of saprotroph（P<0.05）， whereas DL enhanced the number of symbiotroph（P<0.01）.The effects of the treatments of litter on were associated with the physicochemical factors in soil， with the content of total nitrogen and ammonium nitrogen in soil being key factors affecting the differences in the structure of fungal community in soil at the level of phylum and genus， respectively.It is indicated that the input of organic carbon in wetlands can indirectly regulate the structure and functional taxa of fungal community in soils by altering the availability of nitrogen in soils.It will provide a theoretical basis for predicting the potential of carbon sequestration in Calamagrostis angustifolia wetlands and informing strategies for managing ecosystems under the scenarios of climate changes in the future.