Tobacco mosaic virus （TMV） is the most common and significant virus infecting tobacco. Tobacco was treated with potassium glycyrrhizinate， glycyrrhizic acid diamide， glycyrrhetin， sodium cholate， and aluminum reagent to screen novel immune elicitors against TMV. TMV was inoculated after 24 hours of treatment. Quantitative PCR and Western blot were used for detection after 7 days. The results showed that treating tobacco with 10 mg/mL sodium cholate effectively inhibited the expression of TMV gene and the accumulation of TMV-coating protein（CP）， with an optimal induction time of 24 hours. A second sodium cholate treatment on the 11th day post-TMV inoculation sustained induced TMV resistance for over 30 days.The results of analyzing the expression of genes related to the JA pathway， SA pathway， and ethylene pathway showed that sodium cholate-induced TMV resistance is closely associated with the JA signaling pathway. It is indicated that sodium cholate is an effective immune elicitor against TMV and promotes the growth of tobacco seedlings.