A new technology called sugarfree tissue culture technology was used to increase the quality of potato testtube seedlings and optimize the production,and to improve the technical system of virusfree seed potato breeding. The virusfree testtube seedlings of earlymaturing potato cultivar “Huashu 1” and the traditional MS medium with 4% sucrose was used as the test and control (CK) to compare two different culture methods. The effects of different media,supports and inoculation methods on the growth and development of potato plants in sugarfree tissue culture were studied. The results showed that the testtube seedlings under sugarfree tissue culture grew faster and were more robust,and had more advantages in the production of virusfree potato seedlings compared with the traditional tissue culture. Under sugarfree tissue culture conditions,further removal of organic components from MS medium,only containing inorganic components,had no significant impact on the growth of virusfree seedlings,but more effective in reducing pollution. The results of using vermiculite as support instead of agar showed that the virusfree seedlings supported by vermiculite grew faster,had longer internodes and showed signs of “elongation”. The results under the condition of vermiculite as support culture showed that the seedling growth of doublesegment cutting was significantly better than that of singlesegment cutting. The survival rate of doublesegment cutting was higher,and the segment was larger and easy to operate. The testtube seedlings under sugarfree tissue culture were further cutting into the net room to produce minitubers,of which the number and size had no significant changes compared with that from testtube seedlings under the traditional sugar culture. The new sugarfree tissue culture technology shortens the cultivation period of potato seedlings,reduces the production cost,simplifies the cutting propagation procedure of minituber production. It will lay a foundation for the realization of mechanized cutting of potato seedlings.