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32 华 中 农 业 大 学 学 报 第 43 卷

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Mechanisms of interfacial interactions for bacterial
adhesion in rhizosphere

CHEN Yanzhao,XU Shulin,QU Chenchen,ZHANG Ming,
GAO Chunhui,WU Yichao,DAI Ke,HUANG Qiaoyun,CAI Peng
College of Resources and Environment/National Key Laboratory of Agricultural
Microbiology，Huazhong Agricultural University，Wuhan 430070，China

Abstract Interfacial reaction is the foundation and prerequisite for bacteria to adhere，colonize，form
biofilms，and perform ecological functions in the environment. It is of great significance for plants to absorb
nutrients and antagonize pathogenic microorganisms. Most studies on the microbe-plant rhizosphere interac⁃
tions are conducted from the perspectives of ecology and molecular biology，using multi-omics methods to
study the effects of root exudates on the quantity of colonization，the composition of community，and the
physiological functions of rhizosphere microorganisms. However，the physical and chemical interfacial mech⁃
anisms involved in bacterial colonization and their contributions to adhesion have been ignored. This article
reviewed the regulatory effects of different types of root exudates on the properties of bacterial surface，the
molecular composition of extracellular polymeric substances （EPS），and the function of adhesion in term of
the mechanisms of interfacial interactions. The main modes and micro mechanisms of the interaction be⁃
tween bacteria and plant bio-macromolecules during the process of adhesion were summarized. The visual⁃
ization methods for studying the process of rhizosphere colonization and methods for analyzing the interac⁃
tion between microorganisms and plant bio-macromolecules were discussed. The directions of studies that
urgently need to be strengthened including analyzing the biomolecular composition of rhizosphere，predicting
the function of adhesion protein，and the in situ methods for observing the colonization of rhizosphere were
proposed.
Keywords interfacial reaction； rhizosphere colonization； root exudates； extracellular polysaccha⁃

rides； adhesion proteins

（责任编辑：葛晓霞）