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30 华 中 农 业 大 学 学 报 第 43 卷

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Effects of associated cultivation on soil fertility and microbial

diversity in rhizospheres soil of main tomato

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1
YANG Da ,PANG Shichan ,LI Zujian ,SONG Jingjing ,YANG Shangdong 1
1.College of Agricultue/National Experimental Teaching
Demonstration Center of Plant Science， Guangxi University ，Nanning 530004， China；
2.Guangxi South Subtropical Agricultural Science Research Insiture，Chongzuo 532415， China；
3.Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation /
College of Marine Sciences，Beibu Gulf University， Qinzhou 535011， China

Abstract The associated cultivation modes of tomato and lettuce， amaranth， cabbage， mint and on⁃
ion were set up. The effects of different associated crops on the fertility of the rhizosphere soil and the diver⁃
sity of bacteria and fungi of the main tomato were analyzed based on high-throughput sequencing technolo⁃
gy to study the mechanism of the effects of different associated crops on the fertility and health of the rhizo⁃
sphere soil of the main tomato， and provide theoretical basis and technical support for constructing the eco⁃
logical cultivation model of tomato. The results showed that the associated cultivation with four associated
crops except of cabbage significantly increased the activity of soil enzymes including aminopeptidases and
phosphatase， and microbial biomass carbon， nitrogen and phosphorus in the rhizosphere soil of the main to⁃
mato plant. Although the associated cultivation did not enrich the microbial diversities in the rhizosphere soil
of the main tomato plant， it changed the microbial abundance ratio in the rhizosphere soil of the main toma⁃
to plant. At phylum level， the associated cultivation increased the abundance ratio of Proteobacteria， Fir⁃
micutes and Patescibactera bacteria. At genus level， norank f. Microscillaceae， norank_f. norank. o. Vicin‑
amibacterales， Bacillus， norank.f. Gemmatimonadaceae， and Altererythrobacter were the top ten unique
dominant bacteria in rhizospheres of tomatoes associated with different crops. In addition， the associated
cultivation changed the diversity and abundance of fungi in the rhizosphere soil of the main tomato plants to
varying degrees， and enriched the dominant fungi of the phylum Chlamydomonas， Basidiomycetes， and
Phyllomycetes. At the same time， it enriched the dominant fungi including unclassified o Sordariales， Ol‑
pidium， and Conoxybe， Phallemonium， unclassified o Pleosporales， unclassified o Boletales， and Sodio‑

myces， and increased the abundance ratio of Chaetomium as well. It is indicated that the associated cultiva⁃
tion of lettuce， amaranth， mint and onion in tomato contributes to improve the fertility of tomato rhizo⁃
sphere soil and maintain the health of rhizosphere microenvironment soil in varying degrees. Among them，
the effect of associated cultivation of lettuce and mint on improving soil fertility and maintaining soil health
is better than that of corresponding cabbage， amaranth and onion.
Keywords tomato（Solanum lycopersicum L.）； associated cultivation； rhizosphere； soil fertility；mi⁃
crobial diversity
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