Page 32 - 《华中农业大学学报（自然科学版）》2022年第6期

26 华 中 农 业 大 学 学 报 第 41 卷

and nitrogen microbial community in rice tanker farming［J］.En‐ 2021，52（7）：1869-1876.ZHAO C M，WANG W B，ZHANG Y
vironmental science and technology，2021，44（10）：27-35（in F，et al.Structural characteristics of soil fungal communities in dif‐
Chinese with English abstract）. ferent parent rubber forests and their correlation with soil environ‐
［39］ 赵春梅，王文斌，张永发，等 . 不同母质橡胶林土壤真菌群落结 mental factors［J］. Journal of south China agriculture，2021，52
构特征及其与土壤环境因子的相关性［J］. 南方农业学报， （7）：1869-1876（in Chinese with English abstract）.

Effects of application rates of nitrogen on rice yield，
carbon and nitrogen，microbial community in soil under

mixed sowing of green manure

HU Qiliang,YANG Binjuan,LIU Ning,HUANG Guoqin

Center for Ecological Sciences，Jiangxi Agricultural University，Nanchang 330045，China

Abstract Five treatments including no nitrogen application （CK），conventional nitrogen application
（MRN）and reduced nitrogen application by 20%，40% and 60%（MRN1，MRN2 and MRN3）under the condi‐
tion of mixed sowing of Chinese milk vetch and rapeseed were used to study the effects of mixed sowing of green
manure and combined application of nitrogen fertilizer on the rice yield，the carbon and nitrogen and microbial
community in soil，and the main environmental factors affecting microbial community structure.The rice yield
and its components，the carbon composition，the nitrogen composition and relative abundance of microbial com‐
munity in soil were measured.The effects of carbon and nitrogen on microbial community were analyzed.Results
showed that the yield of early rice and late rice increased by 24.42%-39.23% and 19.34%-31.59% compared
with CK treatment.The effect of MRN1 treatment on early rice and late rice was the best.Compared with CK，
the content of nitrate nitrogen，ammonium nitrogen and alkali-hydrolyzable nitrogen in MRN1 was significantly
（P<0.05）increased by 22.07%，19.05% and 11.20%，respectively.The content of soluble organic carbon and
easily oxidized organic carbon in soil increased with the decrease of nitrogen application.The content of nitrate ni‐
trogen and alkali-hydrolyzable nitrogen in soil decreased with the decrease of nitrogen application rate.The domi‐
nant bacteria were Chloroflexi，Proteobacteria，Acidobacteriota，Actinobacteriota，Firmicutes.Applying nitrogen
fertilizer increased the evenness index of bacterial community.The content of alkali-hydrolyzable nitrogen，nitrate
nitrogen and ammonium nitrogen was negatively correlated with dominant flora.The content of microbial nitro‐
gen，easily oxidizable organic carbon and soluble organic carbon was positively correlated with dominant flora.
The environmental factors having the greatest influence on the bacterial community structure were alkali-hydro‐
lyzable nitrogen，microbial biomass nitrogen and soluble organic carbon.It is indicated that the mixed sowing of
green manure and nitrogen reduction by 20% increase the rice yield and the content of inorganic nitrogen in soil，
the community abundance of dominant bacteria，which is conducive to the reduction of emissions and the increase
of efficiency in paddy fields，and the maintenance of the stability of ecosystem in paddy field.
Keywords mixed sowing of green manure back to the field；rice yield；nitrogen reduction；soil car‐
bon and nitrogen；microbial community；ecosystem balance of paddy field

（责任编辑：张志钰）