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第 ３ 期 吴佳 等:甘蔗鞭黑粉菌致病力差异菌株转录组分析 ５ ９

htt p s :// doi．or g / １０．１３７１ / annotation / ed４d１４７３Ｇcf８０Ｇ４e８５Ｇad９dＧ ２０１Ｇ２５３．
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Anal y zin g transcri p tomeofS p orisoriumscitamineum
strainswithdifferent p atho g enicit y

,
,
WUJia , LIHuizhon g DENG Quan q in g CHENJianwen , SHEN Wankuan
/
Colle g eo fA g ronom y Ministr yo fA g ricultureandRuralA ff airs , Scienti f ic
Observin gandEx p erimentalStationo fCro pCultivationinSouthChina ,
SouthChinaA g riculturalUniversit y Guan g zhou５１０６４２ , China
,
Abstract Illuminase q uencin g technolo gywasusedtoconstructthecDNAlibrar y oftwoS p orisoriＧ

),
)
umscitamineum strainsSs１６ ( stron gp atho g enicit y andSs４７ ( weakp atho g enicit y followedb ytranＧ
,
scri p tomese q uencin gandbioinformaticsanal y sisafter q ualit ycontrolinordertoinvesti g atethedifferＧ
encesattranscri p tionallevelbetweenS．scitamineum strainswithdifferentp atho g enicit yanddiscover
p atho g enicit y Ｇrelatedg enes．Theresultsshowedthatatotalof８．３１G ( Ss１６ ) and９．８８G ( Ss４７ ) cleanreads
wereobtained．Therewere６４９differentiall yex p ressedg enes ( DEGs ) includin g２９９u p Ｇre g ulatedg enes
and３５０downＧre g ulated g enesbetweenSs１６andSs４７．ResultsofGOannotationandKEGGp athwa y enＧ
richmentanal y sesshowedthatDEGsweresi g nificantl yenrichedinKEGGp athwa y sinvolvedinstarch
andsucrosemetabolism , ABCtrans p orters , de g radationofaromaticcom p ounds , metabolic p athwa y s , uＧ
p
rinemetabolism．These p athwa y saremostl y relatedto p roteinbios y nthesis , trans p ortandmetabolismof
substances．Itwill p rovidemolecularbasisforrevealin g thep atho g enicmechanismofS．scitamineum．
Ke y words su g arcane ; S p orisoriumscitamineum ; transcri p tome ; atho g enicit y Ｇrelatedg enes ; difＧ
p
ferentiall yex p ressedg enes ( DEGs ); bioinformaticsanal y sis

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