Page 45 - 《华中农业大学学报（自然科学版）》2024年第1期

第 1 期 覃欣仪 等：伴生不同作物对主栽番茄植株根系内生微生物群落结构的影响 39

Effects of different associated crops on endophytic microbial
community structure in tomato roots

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QIN Xinyi ,HE Lizhen ,XIAO Kezhuo ,PANG Shichan ,SONG Jingjing ,YANG Shangdong 1
3
1.National Experimental Teaching Demonstration Center of Plant Science/
College of Agriculture，Guangxi University，Nanning 530004，China；
2.Guangxi South Subtropical Agricultural Research Institute，Chongzuo 532415，China；
3.Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation，
College of Marine Sciences，Beibu Gulf University，Qinzhou 535011，China

Abstract The effects of different associated crops on the structure of endophytic microbial communi⁃
ty in tomato roots were analyzed to study the diversity and structure of endophytic microbial community in
tomato roots， and to explore and develop the functions of beneficial microorganisms. Five associated crops
and monoculture treatments including tomato associated lettuce（A）， amaranth（B）， cabbage（C）， chive
（D）， mint（E） and monoculture（F） were set under the same conditions. The structures of endophytic mi⁃
crobial community in tomato roots were analyzed with MiSeq high-throughput sequencing technology. The
results showed that Streptomyces， Burkholderia-Caballeronia-Paraburkholderia， Devosia， Nocardioides

and Massilia were the top five common dominant endophytic bacterial genera in roots of tomatoes under as⁃
sociated and monoculture systems. However， Noviherbaspirillum was the special dominant endophytic bac⁃
terial genus in roots of tomatoes under tomato companioned lettuce （A） associated system； Meanwhile，
Actinoplanes was also the unique dominant endophytic bacterial genera in roots of tomatoes under tomato
companioned amaranth （B） associated system. Moreover， Herpetosiphon was the special dominant endo⁃
phytic bacterial genera in roots of tomatoes under tomato companioned chive （D） associated system.Fur⁃
thermore， Microbacterium was the special dominant endophytic bacterial genus in roots of tomatoes under
tomato companioned mint （E）. By contrast， Bacillus， Marmoricola and Glycomyces were the unique dom⁃
inant endophytic bacterial genera in roots of tomatoes in monoculture system（F）. In addition， Olpidium，
unclassified_o_Sordariales and unclassified_p_Ascomycota were the common dominant endophytic fungal
genera in roots of tomatoes under associated and monoculture systems. And lmmersiella was the special
dominant endophytic fungal genera in roots of tomatoes under tomato companioned lettuce （A） associated
system； Meanwhile， Candida， unclassified_c_Agaricomycetes， Phialemonium were the unique dominant
endophytic fungal genera in roots of tomatoes under tomato companioned amaranth （B）； Geminibasidium，
Plectosphaerella and Rhizophagus were the unique dominant endophytic fungal genera in roots of tomatoes
under tomato companioned cabbage （C）； unclassified_p_Chytridiomycota， unclassified_f_Ceratobasidiace‑
ae， Ceratorhiza， unclassified_o_Boletales， and unclassified_p_Basidiomycota were the special dominant
endophytic fungal genera in roots of tomatoes under tomato companioned chive （D）； By contrast， Gibber‑
ella was the unique dominant endophytic fungal genera in roots of tomatoes under monoculture system
（F）. It is indicated that the compositions of dominant endophytic bacteria and fungi at phylum or genera
level in tomato roots can be altered by associated cultivation with other crops in comparison with the mono⁃
culture of tomato. The special dominant endophytic bacterial and fungal genera can be enriched by associated
with other crops. The composition of specific dominant endophytic microorganisms enriched by associated
cultivation has the potential to enhance the resistance of tomato to environmental biological or abiotic stresses.
Keywords tomato； associated cultivation； root system； endophytic microorganism； continuous crop⁃
ping obstacle； environmental stress
（责任编辑：张志钰）