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第 1 期                      陈俊鹏 等:121 份小麦地方品种 HMW-GS 组成分析                                   23

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                          Compositions of HMW-GS in 121 wheat landraces in China



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                   CHEN Junpeng ,PENG Min ,WANG Xiaofang ,TANG Qingyi ,ZHANG Daorong ,REN Xifeng         1
                         1.College of Plant Science and Technology/Hubei Hongshan Laboratory, Huazhong
                                         Agricultural University, Wuhan 430070, China;
                         2.Shiyan Academy of Agricultural Sciences, Hubei Province,Shiyan 442000 , China;
                      3.Xiangyang Academy of Agricultural Sciences, Hubei Province,Xiangyang  441057 , China


                   Abstract  The  compositions  of  HMW-GS  in  121  wheat  landraces  in  China  were  identified  and  ana‐
               lyzed  with  SDS-PAGE  to  mine  high-molecular-weight  glutenin  subunits (HMW-GS)  combinations  with
               application value and screen wheat landraces with high-quality. The results showed that 3, 9 and 6 types of
               allelic variations were detected in the materials tested at the  Glu-A1, Glu-B1 and Glu-D1 locus, respec‐
               tively. Among them, null, 5+10, and 2+12 had the highest frequency at the Glu-A1, Glu-B1 and Glu-

               D1  locus,  with  the  frequency  of  78.51%,  76.86%,  and  76.03%,  respectively.  There  were  30  combina‐
               tions of subunit formed by different types of allelic variation, among which the combination type of null/7+
               8/2+12  subunit  had  the  highest  proportion,  accounting  for  55.37%.  However,  the  quality  score  of  this
               combination type of subunits was relatively low, with 6 points only. The combination types of subunits in‐
               cluding  1/7+8/5+10,  1/7+9/5+12,  1/7+9/5+10,  2  */7+8/5+10,  and  2  */7+9/5+12  had  the
               highest  score  and  became  high-quality  combinations  of  subunits.  Wheat  landraces  with  these  high-quality
               combinations  of  subunits  including  Jiangmai,  Qianjiaomai  and  Bianguangtoukemai,  etc.  ,can  be  used  as
               elite genetic resources for improving the quality of modern wheat.
                   Keywords    wheat;  landraces;  high  molecular  weight-glutenin  subunits (HMW-GS);  quality  im‐

               provement; combination of subunits

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