Page 75 - 《华中农业大学学报（自然科学版）》2024年第1期

第 1 期 陆秀红 等：番茄响应南方根结线虫侵染相关转录因子的初步分析 69

sis of bHLH transcription factor and involvement in the infec⁃ cum）［J/OL］.BMC genomics，2015，16（1）：39［2022-12-08］.
tion by yellow leaf curl virus in tomato （Solanum lycopersi‑ https：//doi.org/10.1186/s12864-015-1249-2.

Preliminary analysis of transcription factors associated with tomato

response to infection of Meloidogyne incognita

1
1
1
2
LU Xiuhong ,HUANG Jinling ,QIN Liping ,LIU Zhengrong ,LIU Zhiming 1
1.Institute of Plant Protection， Guangxi Academy of Agricultural Sciences/
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South
China Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for
Crop Diseases and Insect Pests，Nanning 530007， China；
2.College of Agricultural， Guangxi University， Nanning 530005， China

Abstract In order to explore the gene expression pattern at transcriptional level of tomato after inocu⁃
lated with Meloidogyne incognita， transcriptome sequencing was performed on tomato root at 6 h， 12 h，
24 h and 48 h after inoculated with 2nd instar larvae of root-knot nematode. The key transcription factor re⁃
lated to the tomato response to the infection of M. incognita were analyzed and the sequencing results were
verified by quantitative real-time PCR. The results showed that 350， 390， 580， 1 154 genes were differen⁃
tially expressed at 6 h， 12 h， 24 h and 48 h after inoculation， and 11， 11， 19 and 50 transcription factors
were differentially expressed， respectively. They belonged to 15 transcription factor families， of which
MYB family and bHLH family are the most abundant with 20 genes， followed by ERF family with 19
genes， WRKY family with 15 genes， and bZIP family with 9 genes. Further studies showed that the tran⁃
scription factors of ERF，WRKY，MYB and bHLH family were the most differentially expressed.
Solyc03g005520， Solyc02g094270 and Solyc09g066350 were significantly up regulated and the log 2FC at
48 h after inoculation was 9.16， 6.49 and 6.33， respectively. Solyc03g005520， Solyc02g094270 and
Solyc09g066350 were significantly down regulated， and log 2FC at 48 h after inoculation was -2.60，-1.72
and -1.70，respectively. The results of qRT-PCR analysis showed that the expression trend of six random⁃
ly selected genes was consistent with the sequencing results. The results of this study suggest that the tran⁃
scription factors of ERF， WRKY and bHLH family may be involved in the interaction between tomato and
M. incognita， and play an important regulatory role in tomato response to the infection of M. incognita.
Keywords tomato； transcription factor； Meloidogyne incognita； nematode infection； qRT-PCR

（责任编辑：边书京）