The Role and Function of Aspartate Transaminase of Mesobrorhizobium MAFF303099 in the Symbiotic Nitrogen Fixation

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    In order to find the regulatory genes related to symbiotic nitrogen fixation in rhizobia, homologous recombination was used to knocked out 10 up-regulated genes during the symbiotic nitrogen fixation of Lotus japonicus and Rhizobium MAFF303099. The result shows that the nitrogenase activity of the Δmlr5883 was 40% lower than that of the wild-type MAFF303099, the infected cells morphology did not change significantly, and replenishing mlr5883 can restore nitrogenase activity. It is predicted that mlr5883 encodes an aspartate aminotransferase, and the aspartate aminotransferase activity of this MLR5883 is 16.67U/mg in vitro. The mutation of mlr5883 will affect the efficiency of nitrogen fixation, and it is speculated that it may be involved in the metabolic process of supplying carbon sources by plants.

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  • Received:May 14,2021
  • Revised:June 09,2021
  • Adopted:July 08,2021
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