Effect of Bacillus cereus GD2 on the transcriptome of pine wood Nematode invading host
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S763

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State key R & D Program

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    Abstract:

    In order to further clarify the role of bacteria in pine wilt disease (PWD), Pinus Massoniana was inoculated with Bursaphelenchus Xylophilus (CK), a mixture of Bursaphelenchus xylophilus and the endophytic bacterium GD2(T) respectively. Transcription sequencing, Gene Ontology (GO) and KEGG enrichment analysis were performed on the Bursaphelenchus Xylophilus, and Real-time Quantitative PCR (RT-qPCR) was used to verify the target Gene expression. The results showed that 143 differentially expressed genes (DEGs) were detected, among which 63 were up-regulated and 80 were down-regulated. The analysis of Gene Ontology showed that the differentially expressed genes were enriched in immune system process, cell membrane, metabolic process, transporter activity and other functions. KEGG pathway was enriched in ECM-receptor interaction, Renin?angiotensin system, Insulin signaling pathway and other pathways. These GO functions and KEGG pathways were involved in immune regulation and reproductive development of the body. Eight genes were randomly selected for RT-QPCR verification, and the expression level was consistent with the sequencing results, which proved the accuracy of the sequencing results. The above analysis provides valuable reference for further revealing the molecular mechanism by which bacteria enhance the adaptability of pine wood nematode to its host.

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History
  • Received:February 05,2021
  • Revised:May 07,2021
  • Adopted:July 23,2021
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