The kiwifruits soft rot is the most serious disease during the storage and transportation of kiwifruits, which causing great economic losses. In this study, the pathogenic fungus causing kiwifruit soft rots were isolated and identified by examining the morphological and microscopic characteristics, combined with the results of pathogenicity testing and ITS (internal transcribed spacer) sequencing, and the inhibitory effect of carvacrol on kiwifruits soft rot was further evaulated. Two species, Botryosphaeria dothidea and Phomopsis vaccinii, were identified as the main pathogens causing kiwifruit postharvest soft rots, the other three species followed by Botrytis cinerea, Fusarium proliferatum and Alternaria alternata. Carvacrol had significant antifungal activity against eight soft rot pathogens,especially for B. dothidea and Phomopsis sp.,which half effective concentration (EC₅₀) were 12.57 and 9.09 μL / L, respectively. Simultaneously, carvacrol effectively reduced the incidence of soft rot in kiwifruit under simulatedsales environment, which was only 50.17% compared with the control group, and had no significant effect on fruit quality(P > 0.05). Therefore, carvacrol has great potential to control soft rot of postharvest kiwifruit.