Regulators of G-protein Signaling as a negative regulator of G protein signal transduction pathway, plays an important role in the pathogenicity and sexual reproduction of plant pathogens. Study the relationship between parasitism and RGS protein in plant pathogenic fungi will lay a theoretical foundation for further functional analysis of different types of RGS in different parasitic fungi. forty-nine filamentous fungi with the whole genome sequence were used as research objects. OrthoVenn2 was used to perform RGS homologous gene alignment, online BLASTp alignment based on the 4 RGS amino acid sequences of Saccharomyces cerevisiae, and using keyword search in NCBI database, etc., and through SMART conservative domain analysis, the results showed that there were 229 RGS in 49 fungi, and the number of RGS in each fungus ranged from 3 to 9. According to the conserve domains in RGS, above RGS protein can be divided into DEP-RGS, RGS-TM, PXA-RGS-PX, RGS, RGS-PAS-PAC, TM-RGS and other six types, of which RGS with two special conserved domains, RGS-PAS-PAC and TM-RGS, are mainly concentrated in semi-living vegetative pathogens and dead vegetative pathogens, further genetic analysis of 229 RGS proteins showed that RGS with the same conserved domain was closely related. This study found that plant pathogenic filamentous fungi have six types of RGS proteins, among which the types and quantities of RGS proteins of hemibiotrophic and necrotrophic fungi were higher than that in biotrophic fungi.