Water-soluble nanometer fluorescent carbon points were synthesized in one step by "microwave method", then the silicon dioxide was grafted on the surface of carbon points by “sol-gel” method through tetraethyl orthosilicate (TEOS) as the cross-linking agent and phenyl ether methyl cycloazole as the template molecule. The polymer C-dot@MIP with good application prospects in trace detection of pesticide residues was obtained after the template molecules were washed off. Experimental results showed that the minimum detection limit calculated by 3σ/K was 0.93 μg/mL, and the recoveries of blank and standard spiking sample were not exceeding 25 percent.