QuEChERS pretreatment combined with ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） method was developed and used to determine pesticide residues including phoxim，cypermethrin and spinetoram in T. wilfordii root and its rhizosphere soil to establish the method for detecting the content of three pesticide residues commonly used in the process of planting Tripterygium wilfordii. The sample was soaked with appropriate amount of water and then extracted by acetonitrile ultrasonic，purified by N-propyl ethylenediamine （PSA） adsorption，detected by optimized UPLC-MS/MS method，and quantified by matrix external standard method. The results showed that the detection limit of pesticides in root like matrix was 0.001-0.714 μg/kg，LOQ was 0.002-2.380 μg/kg。The limit of detection （LOD） of three pesticides in root and soil samples was 0.001-0.714 μg/kg and 0.002-0.810 μg/kg，respectively. The limit of quantitation （LOQ） of three pesticides in root and soil samples was 0.002-2.380 μg/kg and 0.006-2.410 μg/kg，respectively. The linear relationship，recovery rate and relative standard deviation were in line with the relevant provisions of detecting pesticide residue. It is indicated that the method established is simple，rapid，sensitive and accurate，and can meet the requirements of rapidly determining three pesticide residues in Tripterygium wilfordii and rhizosphere related soil.